50例骨肉瘤标本p53 p16蛋白表达临床分析 word格式.docxVIP

50 cases of osteosarcoma P53, P16 specimens of protein expression clinical analysisPostgraduate: Sikandaer.SiyitiSupervisor: Prof. Song XinghuaAbstractObjective: P53, P16 gene expression in the mechanism of bone tumors, using immunohistochemistry of bone tumors P53, P16 protein, a preliminary study P53, P16 gene in bone cancer development and the interaction and pathological features of osteosarcoma, biological behavior and clinical significance, and learn Han and minority P53, P16 gene expression in osteosarcoma is different. Methods: Tumor Hospital ofXinjiang Medical University, Department of Pathology, August 2005～August 2010, 50cases of osteosarcoma paraffin specimens, using immunohistochemical SP method for immunohistochemical staining. The group of 27 males and 23 females, aged 8 to 78 years, mean age 30 years old, 15 cases of Han nationality, ethnic minorities, 35 cases (24 cases Uygur, Hui, 7 cases of Kazak 4). All cases were confirmed by clinical history, X ray, and pathology with diagnosis. Results: The group of 50 cases of osteosarcoma osteosarcoma paraffin specimens, P53 protein expression rate was 73.33% (Han), 68.57% (minorities) ; paraffin osteosarcoma osteosarcoma specimens, P16 protein expression were 80% (Han),97.14% (minorities); there was no significant difference (P＞0.5). Conclusion: P53 geneabnormal expression in osteosarcoma and clinical prognosis of occurrence. p16 and prognosis of osteosarcoma.Key words: P53; P16; osteosarcoma; Immunohistochemistry论文独创性说明本人申明所呈交的学位论文是在我个人在导师的指导下进行的研 究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的 地方外，论文中不包含其他人已经发表或撰写过的研究成果。与我一同 工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并 表示了谢意。学位论文作者签名：签字日期： 导师签名：签字日期： 关于论文使用授权的说明本人完全了解学校关于保留、使用学位论文的各项规定， （选择“同意/不同意”）以下事项：1.学校有权保留本论文的复印件和磁盘，允许论文被查阅和借阅， 可以采用影印、缩印或扫描等复制手段保存、汇编学位论文；2.学校有权将本人的学位论文提交至清华大学“中国学术期刊(光 盘版)电子杂志社”用于出版和编入 CNKI《中国知识资源总库》或其他 同类数据库，传播本学位论文的全部或部分内容。学位论文作者签名：签字日期： 导师签名：签字日期： 前言骨肉瘤（osteosarcoma）在我国恶性肿瘤中是最为常见的之一，骨肉瘤的恶性程 度是非常高的，预后也是非常差的，它可以在几个月内就转移至肺部，手术后的 5 年生存率也不超过百分之二十，约有四分之三的骨肉瘤发生在股骨远端