不同材料及等离子体处理硬性角膜接触镜表面细菌粘附能力对比分析-眼科学专业论文 word格式.docxVIP

不同材料及等离子体处理硬性角膜接触镜表面细菌粘附能力对比研究中文摘要硬性角膜接触镜；等离子体表面改性；MTT 比色法；旋涡震荡-菌落计数法；扫描电子显微镜第三部分青少年近视角膜塑形镜配戴者结膜囊及镜盒菌群研究目的研究青少年近视者角膜塑形镜配戴前后结膜囊菌群比较，以及角膜塑形镜存储盒带菌情况。方法分别对101名配戴角膜塑形镜（配戴组），和110名未配戴角膜塑形镜者（对照组），进行结膜囊细菌培养，并对角膜塑形镜存储盒进行细菌培养。所有标本置于37℃ 温箱中培养48h后统计培养阳性率。结果配戴组结膜囊培养菌株59株，阳性率为29.35%，对照组结膜囊培养菌株68株，阳性率为30.90%，两组结膜囊细菌培养阳性率差别无统计学意义（??2=0.12,P0.05）。角膜塑形镜存储盒细菌培养菌株72株，阳性率为45.56%，前三位菌群与结膜囊培养结果一致。三组培养菌群构成示，前三位菌株基本一致，分别是表皮葡萄球菌、金黄色葡萄球菌和棒状杆菌菌属。结论配戴角膜塑形镜不提高青少年结膜囊细菌培养阳性率，镜片及镜盒的清洁可降低镜片污染率。关键词角膜塑形镜；结膜囊；细菌菌群作者：王英明指导教师：张晓峰英文摘要不同材料及等离子体处理硬性角膜接触镜表面细菌粘附能力对比研究Comparison ofinvitrobacterialadherenceto rigid contactlensesofdifferentmaterials andtothecontact lenses withandwithoutplasmasurfacetreatment AbstractPart1Invitrobacterialadherenceto rigid contact lensesofthreedifferentmaterialsObjectiveToobservetheinvitroadherenceabilityofdifferentbacteria(staphylococcusaureus, staphylococcusepidermidisandpseudomonasaeruginosa)todifferentrigidcontactlenses（hexafoconA,enflufoconBandpolymethylmethacrylate）.MethodsContactlenseswereplacedinbacterialsuspension(0.5McF)..Bacteriabindingwas measuredandanalyzedbyMethylthiazolyltetrazolium(MTT)assay,Spiral oscillation-bacterialculturequantitativemethodandscanningelectronmicroscopy (SEM).ResultsMTTassay:AdherenceofstaphylococcusaureustoXOwassignificantlowerthanto EOandPMMA(q=7.379，q=8.207,P0.01),nosignificantdifferencewerefoundbetween EOandPMMA(q=0.828，P0.05).Adherenceofstaphylococcus epidermidistoXOandto EO waslowerthantoPMMA(q=14，q=12.8，P0.01),yetnosignificantdifference betweenXOandEO(q=1.2，P0.05).There was nodifferenceofpseudomonas aeruginosaadherence toallthreekinds ofcontactlens(F=2.155,P0.05).Spiraloscillation-bacterialculturequantitativemethods:Adherenceofstaphylococcus aureustoXOwashigherthantoEO,whiletoPMMAwasthehighest(q=11.65,q=28.64, q=16.99,P0.01).Adherenceofstaphylococcus epidermidistoXOwas significantlylower thantoPMMA (q=5.06,P0.05),nosignificantdifferencewasfoundeitherbetweenXO and EO or between EO and PMMA(q=3.29, q=1.77, P0.05). As for pseudomonas不同材料及等离子