黑麦染色体附加对小麦基因组表观遗传影响的研究-生物化学与分子生物学专业论文 word格式.docxVIP

摘要 关，还有一些属于编码蛋白的基因。显然，外源染色体进入后导致的变异会导致 基因表达上的改变，进而影响性状。关键词：小麦-黑麦附加系小黑麦DNA 甲基化EST-SSRAFLPMSAPABSTRACTABSTRACTRye contents many excellent genes to improve wheat genetic background and qualities.It is an effective method to creat wheat-rye addition line to use the valuable genes of rye because monosomic addition lines are improtent genetic resources.The variations of genetic and epigenetic after alien elite genes introduced into cultivated wheat were detected in this study.Two sets of wheat-rye addition lines were detected by EST-SSR(expressed sequence tag-simple sequence repeat),AFLP(amplified fragment length polymorphism) and MSAP(methylation sensitive amplification polymorphism) markers in this study. We got some significative results.1．The EST-SSR makers was used to explore the variations of genetic in Set A.Wefound that there were a certain amount of genome variations between monosomic addition lines and parental wheat. Two kinds of changes were obtained in coding sequences that some sequences eliminated from parental wheat or some additional sequences were obteined in addition lines. But the proportion of sequences elimination is greater than the gain of additional sequences.And the effection is difference between different chromatids of rye.2．To gain more information about the variation of wheat genome in additionline,AFLP makers were used.We have similar results as in EST-SSR analysis that sequences elimination was the principal variation in wheat genome and different chromatids of rye have different effection in Set A.This conclusion was verified by Set B.3．The variations of epigenetic especially genomic DNA methylation after alienelite genes introduced into wheat were detected by MSAP. Two kinds of isoschizomers was used to detect the DNA methylation rate in Set A and Set B.It shown that there were a large number of viarations between addition line and wheat parental in DNA methylation rate include full methylation and semi-methylation rate.This means DNA met