黑逍遥散对老年性痴呆模型大鼠行为学、形态学及酶学的影响-方剂学专业论文 word格式.docxVIP

中文摘要目的：观察黑逍遥散对Aβ25-35诱导AD 模型大鼠行为学、形态学及相关酶学的影响，探讨黑逍遥散防治AD的作用及其机制。方法：采用Aβ25-35海马区注射诱发大鼠脑内神经元损伤，复制大鼠AD模型；利用Morris水迷宫观察AD大鼠行为学变化；利用光镜和电镜观察脑组织形态学变化；比色法测大鼠血清和脑匀浆超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-PX）活性；酶联免疫法（ELISA）测定脑匀浆中乙酰胆碱酯酶（AchE）、胆碱乙酰转移酶（ChAT）活性和单胺氧化酶（MAO）的含量。结果：黑逍遥散对Aβ25-35诱导AD 模型大鼠学习记忆能力具有明显改善作用；能显著提高大鼠血清和脑组织中SOD与GSH-PX活性、降低MDA含量（p0.05）；能显著提高脑组织匀浆ChAT含量，降低大鼠脑组织AchE、MAO活性（p0.05）；能改善老年性痴呆大鼠的脑组织病理损伤。结论：黑逍遥散对AD模型大鼠学习记忆能力和脑组织病理损伤具有明显改善作用，其作用机制可能与抗自由基氧化、调节中枢胆碱能神经元和单胺类神经元活性有关。关键词：黑逍遥散；老年性痴呆；β淀粉样蛋白；行为学；形态学；酶学ABSTACTObjective:ToexplonetheinfluenceofHeixiaoyaosanonpraxiology,morphologyand enzymologyofAlzheimer’sDisease(AD)ratsinducedbyAβ25-35fragmentssoastodiscuss the role andmechanisms involved ofHeixiaoyaosan in theprevention and treatment ofAD.Method:TheinjectionofAβ25-35 intotheHippocampuswasadoptedtodamagebrainneuronto maketherat’s ADmodel.The changeof AD rat praxiologywasobserved byMorris watermaze;brainmorphologywasobservedunderlightandelectronmicroscopes;theserum andbrainhomogenatecontentionofSOD,MDA,GSH-PXwereexaminedbymeansof chemistry chromatometry,andthe activitiesof AchE,ChAT andthecontentofMAOinbrain homogenate weredetected with Enzymelinked immunosorbent assay(ELISA).Result:Heixiaoyaosanobviouslyimprovedthecapabilityoflearningandremembrance ofADratmodelinducedbyAβ25-35 fragmentsandincreasedtheactivitiesofSOD(p0.05) andGSH-PX(p0.05),andreducedthecontentofMDA(p0.05)intheirserumandbrain tissue, increasedthe content of ChAT(p0.05), decreased theactivities ofAchE, MAO(p0.05) inbrainhomogenate,andimprovedthepathologicalchangesoftheseniledementiainrat brain.Conclusion:Heixiaoyaosancanobviouslyimprovetherat’slearningandmemory ability,andthepathologicalchangeofbrainHippocampusaswellas,themechanismsof whichmayberelatedtoanti-freeradicalsandtheregulationoftheactivitiesofcholinergic neurons and monoaminergic neurons.KEY WORDS：Heixiaoyaosan; AD;Aβ; praxiology; morphology;enzymology英文缩略词缩略词Aβ英文全称β-amyloidprotein中文名称β-淀粉样蛋白Aβ25-35AchEβ-amyloidprotein25-35Acetylcholinesteraseβ