KLK10基因表达增强能够降低人舌癌细胞的增殖和侵袭能力.PDFVIP

·1796 · 南方医科大学学报（J South Med Univ ） 2012;32(12) 基础研究 KLK 10基因表达增强能够降低人舌癌细胞的增殖和侵袭能力 1 1 2 2 郑 红 ，张文玲 ，王笑玉 ，赵国强 1 2 河南大学医学院，河南 开封 475004 ；郑州大学基础医学院，河南 郑州 450052 摘要：目的探讨人组织激肽释放酶10（KLK 10）对舌癌Tca8113 细胞增殖和侵袭力的影响。方法运用分子生物学技术，构建真 核表达载体pIRES2-EGFP-KLK10，转染Tca8113 细胞，分别用RT-PCR、Western blot 检测KLK 10 mRNA 及蛋白表达水平。 MTS 细胞生长实验检测细胞增殖的变化；Transwell 小室细胞侵袭实验检测细胞侵袭能力的变化。结果 pIRES2-EGFP-KLK10 转染Tca8113 细胞获得稳定细胞株，与空白对照组及空载体组比较，KLK 10 mRNA 及蛋白表达水平均明显增高，KLK 10增强表 达的舌癌细胞增殖和侵袭能力均明显减弱（P0.05）。结论 KLK 10表达增强能够降低人舌癌细胞的增殖和侵袭能力。KLK 10 基因可能成为肿瘤治疗上有前途的分子靶点。 关键词：KLK 10；Tca8113；细胞增殖；细胞侵袭 中图分类号：R73-3 文献标志码：A 文章编号：1673-4254（2012）12-1796-04 doi: 10.3969/j.issn. 1673-4254.2012.12.024 Enhancement of kallikrein-related paptidase 10 expression attenuates proliferation and invasiveness of human tongue cancer cells in vitro 1 1 2 2 ZHENG Hong , ZHANG Wenling , WANG Xiaoyu , ZHAO Guoqiang 1Medical College of Henan University, Kaifeng 475004, China; 2 School of Basic Medical Science, Zhengzhou University, Zhengzhou 450052, China Abstract: Objective To study the effect of kallikrein-related paptidase 10 (KLK10) on the proliferation and invasiveness of human tongue cancer cell line Tca8113. Methods The eukaryotic expression vector harboring KLK10 gene (pIRES2-EGFP-KLK10) was transfected in Tca8113 cells and the stable cell lines were selected by G418 screening. The mRNA and protein expression of KLK10 in transfected Tca8113 cells were assayed by RT-PCR and Western blotting, respectively, and the proliferation and invasiveness of the cells were evaluated by MTS cell growth assay and Transwell chamber invasion experiments. Results A stable Tca8113 cell line with hig