芡实壳三萜类提取物降血糖及其改善胰岛素抵抗作用研究-食品科学专业论文 word格式.docxVIP

质。这些活性物质可以改善高血糖小鼠糖代谢、脂代谢紊乱，使高血糖小鼠体重减轻、器官肿大的症状有所缓解，血清胰岛素和C肽水平趋于正常，提高胰岛素敏感水平，增加机体的抗高血糖能力，从而起到治疗高血糖和改善胰岛素抵抗的作用。同时，芡实壳三萜类提取物对PI-3K、PKB等胰岛素抵抗相关基因和蛋白的促进作用以及对Fox01转录因子相关基因和蛋白的抑制作用也得到验证，揭示了芡实壳三萜类提取物可以调控胰岛素抵抗相关多分子靶点转录和翻译水平的表达。关键词：芡实壳；富含三萜；降血糖；胰岛素抵抗Hypoglycemicactivityandregulativemechanismson insulinresistancebytriterpenoid-richextractsfrom EuryaleferoxSalisb.shellsABSTRACTEuryaleferoxSalisb.isamatureseedofEuryalegenusofNymphaeaceae.Itis originproducedinEastAsiaandIndia.ItismainlyproducedinHenan,Ahhui, Hubei andotherplacesinChina.Itisusedasmedicinalplantsoftreatmentforsome chronicdiseases,whichhasahighpharmacologicalvalue.Theouterpackageof euryaleferoxseedsisahardshell.Intheprocessingoffoodindustrialproduction, theeuryaleferoxshells(ES)arealargenumberofabandoned,whichcausedawaste ofshellsresources.Thehypoglycemicandmolecularmechanismsoftriterpenoid- richextractsfromESisanalyzedinstreptozotocin-induced(STZ)diabeticmice, whichisprovidedtheexperimentalbasisfordevelopmentandutilizationofES.Thebrokenshellsweregroundintofinepowderandsoakedin70%ethanolat 65?Cfor16hfortheextraction.Theconcentratedextractwasthendilutedwith water,andthesolutionwasextracted3timeswithpetroleumether,ethylacetateand water-saturatedbutanol,inthatorder.TodeterminethecontentoftriterpenoidinES bySpectrophotometryandinvestigatetheconstituentsoftriterpenoidinextractsby highperformanceliquidchromatography-massspectrometry(LC-MS).Bodyweight, organweightindexandlipidprofileparameterswereobservedinnormaland diabeticmice.Thehypoglycemicactivitywasassessed byfastingbloodglucose (FBG)andfastinginsulin(FINS)tocalculatetheinsulinsensitivityindex(ISI).The mRNAexpressionofFox01,PI-3K,PKB,GLUT2wereexaminedbyRT-PCRand proteinlevelintheliverweredeterminedusingenzymelinkedimmunosorbentassay. Inaddition,thepotentiallyregulativemechanismsoninsulinresistance(IR)were discussed.TheresultsshowedthatthetotalmassfractionstriterpenoidinESextractswas 36.7%,whichwasdeterminedusing5%vanillin-aceticacidandperchloricacidas colorsystem.LC-M