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1778 中草药 Chinese Traditional and Herbal Drugs 48 9 2017 5 1 1 1 1, 2 1, 2 1, 2 1, 2* 江敏红 ，黄松涛 ，胡 ，朱龙平 ，尹 胜 ，赵志敏 ，王冬梅 1. 510006 2. 510006 HSCCC HSCCC K--3 25850 r/min2.2 mL/min277 nm HSCCC HSCCC 1 HSCCC 2.48 g 70%3.01%68 mg 96.2% 2.74% R284.2 A 0253 - 2670(2017)09 - 1778 - 06 DOI: 10.7501/j.issn.0253-2670.2017.09.013 Isolation and preparation of leonurine from Leonurus japonicus by high speed countercurrent chromatography 1 1 1 1, 2 1, 2 1, 2 JIANG Min-hong , HUANG Song-tao , HU Yue , ZHU Long-ping , YIN Sheng , ZHAO Zhi-min , WANG Dong-mei1, 2 1. School of Pharmaceutical Sciences, Sun Yat-set University, Guangzhou 510006, China 2. Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China Abstract: Objective To establish a simple and effective method for the isolation of leonurine from Leonurusjaponicus by high speed countercurrent chromatography (HSCCC). Methods The extraction conditions of leonurine were optimized by one-factor experimental design. After comparing several different solvent systems, the two phase system of ethyl acetate-n-butanol-water (32 5) was finally chosen as operating solvent of HSCCC for the separation of leonurine, in which the lower phase was determined as the mobile phase and the upper phase as stationary. The detection of eluates was performed with an ultraviolet detector at 277 nm. The rotation speed was adjusted at 850 r/min, and the flow rate was 2.2 mL/min. Results Leonurine was successively isolated from n-butanol fraction by HSCCC, and the above established method was also successively applied to the crude extact of L .japonicus . Finally, 68 mg of leonurine with