垂体后叶素对蟾蜍离体心脏的致心衰作用.docVIP

垂体后叶素对离体蟾蜍心脏收缩力的影响 张玲 储莉 何丽亚 徐永俊 [摘要] 目的：探讨垂体后叶素对蟾蜍离体心脏的作用。方法：斯氏法游离蟾蜍心脏，用1ml任氏液维持蟾蜍心脏的正常活动，分别加入含有垂体后叶素（0.125u、0.25u、0.5u/ml）的任氏液，记录心肌收缩力和心率的变化；用0.25u的垂体后叶素诱导的心衰后，将0.04mg的西地兰加入灌流液中，观察其变化。结果： 垂体后叶素可使蟾蜍心脏心肌收缩力降低（与药前比P0.01）；西地兰不能对抗其作用。结论：垂体后叶素可诱发蟾蜍心脏发生心衰，西地兰不能对抗其作用。 [关键词] 垂体后叶素；心衰；蟾蜍；西地兰 Effect of pituitrin on myocardiac contractile force in toads Z HANG ling ,CHU Li ,HE Li-ya ,XU Yong-jun [Abstract] Objective: To explore the mechanism of pituitrin on heart failure in toads and observe the therapeutic effect of deslanoside Methods: Ringers solution was used to maintain the normal action of the heart, then the heart was perfused by Ringers solution 1ml containing pituitrin (0.125u/ml,0.25u/ml,0.5u/ml) and washed before adding the next dosage .The myocardiac contractile force and heart rate were recorded separately after adding each dosage. The other part of this experiment was that the deslanoside was added to the solution when the heart failure model was caused by pituitrin [0.25u/ml]. The parameters were take down. Results: The myocardiac contractile force was reduced by pituitrin(compared with control, P0.05). The reduction of myocardiac contractile force can’t be antagonized by deslanoside. Conclusion: The heart failure can be caused by pituitrin and can’t be antagonized by deslanoside. [Key words] pituitrin； heart failure； toad ；deslanoside 垂体后叶素是由下丘脑神经元合成的神经激素, 在实验研究中常用来诱发冠脉收缩导致心肌缺血，间接引起心肌损伤[1][2]。另有资料表明，充血性心衰患者血浆中血管升压素水平升高并通过V1受体使阻力血管收缩，增加循环阻力；影响心脏收缩，用V1受体阻断剂可改善心脏功能[3]。本实验主要探讨垂体后叶素对心肌的直接作用并观察西地兰对其至心衰的影响。 1 材料与方法 1.1药物与仪器 垂体后叶素（上海禾丰药业公司，批号020601），西地兰（上海海普药业公司，批号030101） ,二道生理仪（成都仪器厂，LMS-2B型） 1.2动物 中华大蟾蜍，雌雄兼用，20只，由蚌埠医学院实验动物科提供。 1.3方法 斯氏法游离蟾蜍心脏并插管，注入1ml任氏液，用试管夹将蛙心插管固定，再用蛙心夹夹住心尖，接张力感受器，并与二道生理仪相接，记录心脏搏动曲线，仪器定标1g=9mm作为对照 垂体组：将蛙心管内1ml 任氏液抽出，注入1ml任氏液中分别含有0.125u/ml、0.25u/ml、0.5u/ml垂体后叶素；西地兰组：将蛙心管内的1ml任氏液抽出，注入1ml任氏液中含有0.25u/