向日葵菌核病生防菌的筛选鉴定及其综合防治-植物病理学专业论文.docxVIP

Screening and Comprehensive Prevention of an Antagonistic Bacterium Strain of Sclerotinia sclerotiorum of sunflowerAbstractSunflower rot disease is a worldwide disease caused by Sclerotinia sclerotiorum (Sclerotinia sclerotiorum (Lib.) de Bary), hosts a wide range plant and causes serious harm. In this study, 30 rhizosphere soil samples from the region of Inner Mongolia, like Hohhot, Baotou City, Wulanchabu Bayannur League, Chifeng City, Tongliao City, Hebei Province, and other area cities and counties with plant tissues, sclerotinia sclerotiorum biocontrol strains isolated from the sample and its control effect were designed to study the theoretical basis and research basis for the biological control of Sclerotinia. Experimental results show that:30 rhizosphere soil samples collected from various regions, after separation screening over 1300 strains of the bacterial strains, better antagonistic effect of a total of 25 bacterial strains of bacteria, accounting for 1.916%. The strain of CF4-51 antibacterial activity is the best and the inhibition zone up to 3.2cm. Inhibitory activity of CF4-51 strain in vitro leaves is 85.33%, pot and field control effect are 78.36% and 64.58% respectively.The bacterial morphological observation and physiological and biochemical characteristics, combined with 16S rDNA sequence analysis, the strain of CF4-51 was identified as Bacillus cereus (B. cereus.). Strain of CF4-51 in sunflower rhizosphere colonization of dynamic experiments, can be colonized in the sunflower rhizosphere and root surface succeed. In addition, biocontrol strains of CF4-51 also good in the Sunflower body colonization, from the sunflower stem base of 5cm can detect strains of CF4-51 exist.Determined the biocontrol bacterial strains culture conditions, the optimum culture conditions strain of CF4-51 was 28℃, initial pH 6, for 48 h up to a maximum amount ofbacteria.Though determination of the antibacterial activity of the volatile substances produced in th