芪连对药对2dmir大鼠糖脂代谢影响的实验研究-中医内科学专业论文.docxVIP

2DM的切入点，对目前清热泻火补气治法在2DMIR临床治疗中的应用现状、发生发展的中医病机进行了阐述，丰富了2DMIR的中医病机学及治法学内容，对寻找有效的治疗方法具有重要意义。2.模型评价：成功复制了实验性2DMIR模型，该实验模型动物不仅表现为高血糖、高血脂、并且表现为部分胰岛β细胞病理切片形态异常，符合2DMIR临床特点。药物治疗后，其检测结果均有不同程度的改善，用以评价药物疗效结果可信。模型可操作性强，死亡率低，成功率高，经济成本适宜，是研究2DMIR的理想载体。本课题从多环节、多靶点研究了芪连对药改善2DMIR的作用机理，丰富了中医药改善2DMIR研究思路和方法，对阐发中药作用机制进行了有益探索。关键词：2 型糖尿病；胰岛素抵抗；黄连黄芪对药；大鼠Experimentalstudy forcoptidisrhizomeandastragaliradixto bigmouse of2DMIRundertheinfluence ofthewith thehigh blood sugarand bloodfats influenceABSTRACTObject：ThistopicisbasedontheclinicalpracticeexperienceofChinesemedicineintreatingtype2diabetesmellitusinsulinresistance(2DMIR),thisresearchpointsoutthatinthetreatmentof2DMIRcanbe“treattheheatwithcold”.Inthisresearch,coptidisrhizome(Huanglianandastragaladixhuangqithetwoherbsarecommonlyusedincombination,whichiscalledmedicinecombination),whichareprovedtobeclinicaleffective,wereselectedasthetreatmentmedicine.Method：Sprague-DawlayfourweekstoinduceIRwiththe highsugarcontentandhighfatcontentfeed，andthenaddingsmalldosageofSTZabdominalcavityinjection.Take the group that using glucophage as control group，randomlygrouping.Aftersuccessfulmousemodeling,eachgroupoftheSDbigmousewererespectivelygivenglucophageqiLianDuiyao(medicinecombinationmadeofcoptidisrhizomeandastragaliradix)smalldosage(equalsto fivetimeofdosagethatusedinthehumanbody)qiLianDuiyaolargedosage(equalstotentimesofdosageusedinhumanbody)andpurifiedwaterperfusioninthestomach.BMI、FBGwererecordedEveryweek，TheTG、HDL-c、FINweremeasured andISI calculated oneweek beforemousemodeling,72hoursaftermousemodeling,andtherightdayofexperimentalconclusionrespectively.Thepancreaswasexcisedandpreservedaftertheexperimentalconclusion.Result：The2DMIRbigmousemodel,Abigmousewaskilledandthepathological sectionwasmadebytheexcisedpancreaticgland.Theotherbigmiceweresuccessfullymadeintomousemodel.Beforemakingthemousemodel,BMI、FBG、TG、HDL-c、FINSandISIbetweeneachgroupdonothavesignificantdifference，Afterthemousemodelingbutbeforeapplyinganydrugstothemodelmice,obviousdifferen