b7h1在肾细胞癌中表达的临床意义及调控机制word格式论文.docxVIP

B7-H1 在肾细胞癌中表达的临床意义及调控机制摘要目的：1.探讨B7-H1分子在肾透明细胞癌组织中表达的临床意义；2.研究IL-2、IFN-α和IFN-γ对人肾透明细胞癌细胞株786-0细胞B7-H1分子 表达的影响。方法：1.采用RT-PCR和免疫组织化学染色检测80例肾透明细胞癌组织及对应的癌 旁肾组织中B7-Hl mRNA和蛋白表达，观察B7-H1表达水平与患者临床病理指标之 间的关系；2.用浓度为1000 U/ml的IL-2、IFN-α和IFN-γ刺激786-0细胞，RT-PCR检测 刺激后786-0细胞B7-H1mRNA的表达，免疫细胞化学和免疫荧光染色检测786-0细 胞B7-H1蛋白的表达，流式细胞术检测刺激后786-0细胞B7-H1分子的表达。 结果：1.肾透明细胞癌组织中B7-H1mRNA表达水平比癌旁组织显著升高(P0.01)， 肾透明细胞癌组织中B7-H1阳性率为41.25%(33/80)，B7-H1的表达水平与患者肿 瘤病理分级相关（P﹤0.05）；2.RT-PCR检测结果显示，IL-2组（0.75±0.06）、IFN-α组（0.68±0.05） 和IFN-γ组（0.95±0.08）的B7-H1mRNA表达量明显高于未经刺激的786-0细胞组（0.30±0.03）（P﹤0.05）；免疫细胞化学和免疫荧光染色检测结果显示，B7-H1 蛋白主要表达在786-0细胞的细胞膜上，IL-2、IFN-α和IFN-γ刺激后表达水平 明显上调；流式细胞术检测显示，B7-H1 蛋白在IL-2组（65.70±3.26）%、IFN-α 组（56.52±1.75）%和IFN-γ组（84.05±3.52）%的表达明显高于未经刺激的786-0 细胞组（20.49±1.03）%（P﹤0.01）。结论：1.B7-H1分子可能成为肾癌早期诊断与预后判断的新指标；IL-2、IFN-α和IFN-γ均上调786-0细胞B7-H1 mRNA和蛋白的表达，其中 IFN-γ上调程度最强，IFN-α上调程度最弱。[关键词] 肾透明细胞癌B7-H1细胞因子肿瘤免疫Clinical significance and regulation of the expression of B7-H1 in renal cell carcinomaAbstractObjective：To analyze the clinical significance of the expression of B7-H1 in clear cell renal cell carcinoma;To study the effects of IL-2,IFN-α and IFN-γ on B7-H1 expression in clear cell renal cell carcinoma 786-0 cells.Methods:1.The B7-H1 mRNA and B7-H1 protein expression in renal cell carcinoma and the nearby renal tissues was determined using RT-PCR and immunohistochemistry assay in 80 patients,the relationship between the expression level of B7-H1 and clinicopathological characteristics was analyzed;2.786-0 Cells were cultured in RPMI-1640 supplemented with 10％ fetal calfserum,The B7-H1 mRNA was detected by RT-PCR and the protein of B7-H1 was detected by cytoimmunochemistry staining、cytoimmunofluorescence staining and flow cytometric analysis. The expression levels of B7-H1 mRNA and the protein ofB7-H1 in 786-0 cells were compared.Results:The B7-H1 mRNA levels were significantly increased in renal cell carcinoma tissues compared with the nearby renal tissues(P0.01); The positive rate of B7-H1 in ren