ctla4ig和抗cd40l单抗对实验性自身免疫性心肌炎影响word格式论文.docxVIP

detection were used to position and qualitative analysis the expression of CTLA4 and CD40L. IFN-γand IL-4 mRNA in muscular tissues were detected by RQ-PCR, sera IFN-γand IL-4 were determined by ELISA.Results:Animal model The incidenee of experimental autoimmune myocardltis was 100%. Body weight of the mice in EAM group and IgG group grew slower than normal control group, even decreased from the second week to the third week.Pathology testing HE staining showed: Myocardial cells in normal control group arranged in uniform. All mice in EAM group and IgG group developed typical severe autoimmune myocarditis, large numbers of inflammatory cells infilatrated all layers of ventricular wall, degeneration and necrosis were also evident. Inflammatorycells significantly decreased after immunologic intervention，there were a few focalinflammatory cells in CTLA4-Ig group and anti-CD40LmAb group, minimal infliammatory cells were observed in CTLA4-Ig+anti-CD40LmAb group. Pathological scores of mice in CTLA4-Ig+anti-CD40LmAb group were significantly lower than CTLA4-Ig group and anti-CD40LmAb group(P0.05), but had no significant difference between CTLA4-Ig group and anti-CD40LmAb group.Myocardical enzymes Sera Tn-I and CK-MB in EAM group and IgG group were markedly higher than those of control group(P0.01). After immunologicintervention ， sera Tn-I and CK-MB significantly decreased(P0.05), those inCTLA4-Ig+anti-CD40LmAb group were lower than CTLA4-Ig group and anti-CD40LmAb group(P0.05), but had no significant difference between CTLA4-Ig group and anti-CD40LmAb group(P0.05).The expression of CTLA4 and CD40L in ventricular tissue There was almost no expression of CTLA4 and CD40L in the cardiac myocytes of normal mice, whereas CTLA4 and CD40L were clearly induced on the infiltrating cells in mice of EAM group and IgG group, CTLA4 and CD40L mRNA were also significantly enhanced. After immunologic intervention, the expression of CTLA4 and CD40L significantly decreased(P