dvcpvn nesfatin1通路对胃牵张敏感神经元放电活动和胃运动调控分析word格式论文.docx

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2018-05-18 发布于上海
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dvcpvn nesfatin1通路对胃牵张敏感神经元放电活动和胃运动调控分析word格式论文.docx

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dvcpvn nesfatin1通路对胃牵张敏感神经元放电活动和胃运动调控分析word格式论文

AbstractObjective:Nesfatin-1asapeptidecanregulategutmotorfunction,buttheunderlying mechanismshaveyettobeelucidated．ThestudyaimedtoillustratetheformationbetweentheDVCandPVNnesfatin-1pathwayandtoexploretheeffectsof nesfatin-1inPVNongastricdistension-sensitiveneuronsandtheregulation bythe DVC.Methods:①Retrograde tracingwere usedtodetermine neuronalprojections. ②Fluorescent-immunohistochemistrywereusedtodetermineNUCB2/nesfatin-1neuronal projections.③Thenucleimicroinjectionand nucleielectricalstimulation, extracellulardischargesofsingleunitneuronwere usedtoobservetheeffectsofnesfatin-1ontheGDneurons.④Gastricmotilityrecordinginvivowereusedto monitortheeffectsofnesfatin-1ontheamplitudeofconstrictionandfrequencyofgastricmotilityinconsciousrats.⑤Gastricmotilityrecordinginvivowereusedtomonitortheeffectsofnesfatin-1ontheamplitudeofconstrictionandfrequencyof gastricmotilityinratswiththevagusnerveto be cutoff．Results:①NUCB2/Nesfatin-1/fluorogold-doublelabeledneuronsweredetectedinthePVN.②Of187recordedneuronsinPVN，126neuronswereGDresponsiveneurons. Injection of nesfatin-1 could excited GD-E neurons(P0.01), and inhibit GD-Ineurons(P0.01). ③Injection ofmixtureofnesfatin-1andSHU9119couldweakentheresponsesinduced bynesfaton-1(P＜0.05).OutofGD-EneuronsinthePVN,24 wereinhibitedbyelectricalstimulation oftheDVC,14wereexcitedand33hadno response. OutofGD-Ineuronsin thePVN, 16 wereinhibited byelectricalstimulation oftheDVC,13wereexcitedand26hadnoresponse.Pretreatmentwith anti-NUCB2/nesfatin-1antibodyinthePVNcouldfurtherdecreasethefiringrateofmostoftheGD-Eneurons(P＜0.01)andincreasethefiringrateofmostoftheGD-Ineurons(P＜0.01).④Gastricmotilityrecordinginvivoshoethatinjection of nesfatin-1inPVN,candecreasedthe amplitudeandfrequency(P＜0.01),SHU9119 couldweakentheresponsesinducedbynesfaton-1(P＜0.05). ⑤Cutoffthevagus nervecouldweakentheresponsesinducedbynesfaton-1(P＜0.05).Conclusion:Nesfatin-1couldinhibitorygastricmotilityinPVNviamelanocortinpathwayand influence thefiringactivityofGDneurons.Postgrad