fasfasl介导胃癌细胞失巢凋亡机制及七方胃痛颗粒干预的研究word格式论文.docx

优秀毕业论文 精品参考文献资料 亡过程。 关键词： 失巢凋亡，七方胃痛颗粒，Fas，FasL，Caspase-8  The research of the mechanism of Fas-FasL signal gastric cancer cells anoikis and Qifangweitong Granule ABSTRACT Objective: To explore the effect on anoikis of Qifangweitong granule -induced SGC-7901 gastric carcinoma cells and its relationship with the expression of Fas, FasL, FADD, Caspase-8 and Caspase-3. Methods: After the 10%、20%、30% concentration of Qifangweitong granule drug serum intervented the anoikis model of SGC-7901 gastric carcinoma cells, Then methyltetrazolium (MTT) assay was performed to determine the growth inhibition of SGC-7901 gastric carcinoma cells）; The apoptosis of SGC-7901 gastric carcinoma cells were evaluated by terminal deoxynucleatidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL); Western Blotting was performed to evaluate the protein expression of Fas, FasL, FADD,Caspase-8 and Caspase-3;The expressions of Fas, FasL,FADD,Caspase-8 and Caspase-3 mRNA were estimated using reverse transcription-polymerase chain reaction (RT-PCR). The levels of Fas, FasL,FADD,Caspase-8 and Caspase-3 were detected by enzyme linked im-munosorbent assay( ELISA). Results: ①Qifangweitong granule inhibited significantly SGC-7901 gastric carcinoma cells proliferation and presented some time- dose dependence（P0.05）;②Qifangweitong granule induced apoptosis in SGC-7901 gastric carcinoma cells was conformed by TUNEL assay, which was in a time- and dose- dependent manne（r P0.05）;③After the 10%、20%、  30% concentration of Qifangweitong granule drug serum intervented the anoikis model of SGC-7901 gastric carcinoma cells, the expressions of Fas,  FasL,FADD, Caspase-8 and Caspase-3 were up-regulated significantly（P 0.05）,which was in a time- and dose- dependent manner. Conclusion: After the Qifangweitong granule drug serum intervented the SGC-7901 gastric carcinoma cells,the expressions of Fas,FasL,FADD, Caspase-8 and Caspase-3 were up-regulated significantl