干旱胁迫下4个野生早熟禾属草种抗旱性研究.docVIP

干旱胁迫下4个野生早熟禾属草种的抗旱性研究 　　摘 要：为筛选出抗旱能力较强的野生草坪种质资源,以早熟禾栽培品种“午夜”为对照，对4个野生早熟禾属草种进行了抗旱性研究。胁迫21 d期间每隔3 d测定叶片相对含水量(RWC)、叶片萎蔫率(LW)、叶片相对电导率(EL)、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的变化。结果表明：随着干旱胁迫天数的增加，RWC含量、SOD和CAT的活性都明显下降，而LW、EL和MDA的含量却明显上升，且各指标之间存在显著或极显著相关性；4个草种抗旱能力强弱依次为硬质早熟禾、草地早熟禾、早熟禾、细叶早熟禾，其中硬质早熟禾与其他材料均有显著差异，抗旱性表现较突出，为选育抗旱草坪草种提供了依据。 　　关键词： 早熟禾属；抗旱；生理反应；干旱胁迫 　　中图分类号： S322;S688.4 文献标识码：A DOI编码：10.3969/j.issn.1006－6500.2011.06.007 　　 　　Resistance of Four Wild-grasses of Poa L. under Drought Stress 　　YAN Li-li 　　(Road and Bridge Department ,Landscape Architecture, Beijing Jiaotong Vocational Technical College, Beijing 102200, China) 　　Ａｂｓｔｒａｃｔ: Taking the variety ‘mid-night’as the CK，the drought stress were studied on 4 wild grasses of Poa L．During the stress period,relative water content(RWC)，electrolyte leakage(EL)，leaf wilting rate (LW )，superoxide dismutase(SOD)，catalase(CAT) and malondialdehyde (MDA) were determined in 3 d intervals．The results showed that drought stresses induced oxidative injury in all cultivars，as demonstrated by the reduction in antioxidant enzymes and increase in lipid peroxidation．Meanwhile it caused RWC to decline，while EL，LW and the content of MDA to increase with proceeding of the treatment. There were significant or very significant correlations during all the idexes for four species.The ranks of drought resistance of four grasses were P. sphondylodes Trin. ，P. pratensis L.，P.annua L. ，P.angustifolia.L. ,and P. sphondylodes Trin. had stronger drought tolerance than others,and were significantly different compared with others.These results would facilitate the selection of drought resistant turfgrasses． 　　Key words: Poa L.；drought resistance；physiological response; drought stress 　　 　　中国有丰富的早熟禾种质资源，约有100多种，以西南、东北地区???多[1]。野生早熟禾宜于潮湿而寒冷的气候，普遍耐寒，但耐旱性较差，并且其野生性状往往不符合大量栽培利用的要求，目前用于建植草坪的早熟禾，主要为国外引进种。而引进种常常不能在中国北方地区得到适应及普及推广，中国北方地区夏季较为干旱是原因之一，干旱胁迫限制影响了草坪草正常生长[2]。所以对本土野生资源的利用显得尤为重要和有意义。 　　为了筛选和培育优良早熟禾品种，本研究以采集的8种（品种）野生早熟禾为材料，在田间试验的基础上，筛选出4