CEBPs在不同发育阶段人毛囊-皮脂腺单位中的表达与意义.docVIP

C/EBPs在不同发育阶段人毛囊-皮脂腺单位中的表达与意义 【关键词】 毛囊-皮脂腺单位 【摘要】 目的 寻找不同发育阶段人毛囊-皮脂腺单位中C/EBPα和C/EBPβ的表达变化规律及与毛囊皮脂腺分化的关系。方法 应用免疫组化方法检测胚胎及新生儿和成人毛囊皮脂腺组织中C/EBPα和C/EBPβ的定位及表达。结果 C/EBPα和C/EBPβ分别在胚胎期毛囊外根鞘，毛乳头，毛母质及皮脂腺细胞的胞核内强表达;新生儿毛囊峡部下端外根鞘基底层细胞的胞核内也存在强表达，基底内层胞核阳性信号逐渐减弱;而成体毛囊核内阳性信号仅定位于峡部下端外根鞘基底层及皮脂腺基底层。毛囊外根鞘内层细胞随分化程度升高，胞浆内C/EBPα和C/EBPβ的表达逐渐升高，毛乳头细胞则未见阳性颗粒。在整个毛囊皮脂腺发育过程中C/EBPα的表达都略强于C/EBPβ。结论 C/EBPα和C/EBPβ在不同发育阶段的毛囊皮脂腺单位中均有表达，定位及表达水平与分化程度密切相关。 关键词 毛囊-皮脂腺单位 C/EBPs 分化 Characteristics of C/EBPs protein expressions in the pilo-sebaceous unit of human at different developmental stage and their biological significance Zheng Yehua，Yang Tian，Gao Qiangguo Department of Cell Biology，Third Military Medical University，Chongqing400038. 【Abstract】 Objective To study the expression of C/EBPαand C/EBPβprotein in human piol-sebaceous uˉnit during different developmental stage.Methods C/EBPαand C/EBPβprotein expressions were studied by using immunohistochemical method.Results C/EBPαand C/EBPβexpressed differently in the outer root sheath，follicular papilla，hair matrix and sebaceous gland in embryonic period.The positive signals were chiefly distributed in the nucleˉuses.The expression of C/EBPαand C/EBPβwere found in the basal cell layer of outer root sheath and sebaceous gland in the new born and maturation phase，The expression in nucleuses of outer root sheat cells decreased with the differentiation but enhanced oppositely in the cytoplasm.Ngetive stain was in the follicular papilla.Conclusion They are expressions of C/EBPαand C/EBPβprotein in human pilo-sebaceous unit at different stage.The expression level is closely correlated with the differentiation degree.This indicates that C/EBPs play important roles in the development of human pilo-sebaceous unit.Key words human pilo-sebaceous unit C/EBPs differentiation C/EBPs蛋白是一组耐热的转录调控因子，能与启动子CCAAT区及多种病毒增强子结合，在细胞增殖、分化、信号转导及肿瘤发生、免疫反应、能量代谢等方面发挥重要作用。有实验表明C/EBP家族在细胞分化，炎症反应及基因调控中扮演了关键角色 ［1］ 。C/EBPs存在于表皮中，与角质形成细胞分化及生长停滞有关 ［2］ 。但它们与毛囊皮脂腺的关系却很少提到。我们检测了C/EBPα、C/