HBsAg阳性孕妇血清乙肝病毒含量与HBV宫内感染关系的研究.docVIP

HBsAg阳性孕妇血清乙肝病毒含量与HBV宫内感染关系的研究 【关键词】 DNA 【摘要】 目的 探讨孕妇外周血中HBV含量与HBV宫内感染的关系。 方法 ELISA法检测孕妇及新生儿外周血HBsAg、HBeAg;FQ-PCR对血清HBV DNA定量检测。 结果 166例新生儿中有28例发生HBV宫内感染（16.87%）;在HBV宫内感染组和非感染组孕妇HBV DNA暴露、各级病毒载量与HBV宫内感染趋势χ 2 检验差异为有统计学意义;HBV DNA 156copies/ml为HBV宫内感染最佳临界预测值，它的敏感度为81.80%，特异度为72.10%，阳性预测值为37.10%，阴性预测值为95.19%。 结论 孕妇外周血HBV DNA阳性不仅是HBV宫内感染的危险因素，且随着孕妇血中乙肝病毒含量的增高，其发生HBV宫内感染的危险性呈现增高趋势;孕妇血HBV DNA156copies/ml为HBV宫内感染最佳临界预测值，孕妇血清中HBV DNA水平对于预测新生儿HBV宫内感染较为重要。 【关键词】 肝炎，乙型;DNA Study on the relationship between HBV DNA content in HBsAg positive pregnant women and the in-trauterine infection SONG Xiu-xia ，LI Tie-gang ，YUAN Chen-li ，WANG Su-ping ，FENG Li-ping ，SHUANG Jie-yu ，QU Yi-qun . Department of Epidemiology，Shanxi Medical Universi-ty，Taiyuan 030001，China;Department of Gynaecology and Obstetrics，Taiyuan Infectious Dis-ease Hospital，Taiyuan 030001，China 【Abstract】 Objective To explore relationship between HBV DNA content in HBsAg positive preg-nant women and the HBV intrauterine infection.Methods HBsAg and HBeAg in pregnant woman and their newborns serum were tested by ELISA，HBV DNA were detected by Fluorescence Quantitative PCR（FQ-PCR）.Results 28of166newborns were infected by HBV.The value of association be-tween the HBV infection rate and virus content in gravidaserum showed statistical significance.The best critical predictive value of HBV intrauterine infection was156copies/ml，the sensitivity，specifici-ty，positive and negative predictive value was81.80%，72.10%，37.10%and95.19%，respectively.Conclusions The risk of HBV intrauterine transmission showed a increasing trend comparing with the HBV DNA content rising in pregnant women.It was very important to comprehend the HBV DNA level in pregnant women to predict HBV intrauterine infection. 【Key words】 Hepatitis B;DNA 据统计，我国慢性乙型肝炎病毒（hepatitis B virus，HBV）携带者已达1.3亿人，30%～50%的病人是通过母婴传播而感染的。新生儿感染乙型肝炎后约90%以上会成为慢性携带者，而慢性HBV感染至成年后，可致肝硬变和肝癌 ［1］ 。据最新妇女健康报告显示，部分携带乙肝病毒的育龄妇女因担心将病毒传给未来的新生儿而不敢怀孕。有关HBV宫内感染影响因素的研究报道称HBsAg阳性孕妇是否致新生儿感染乙肝病