HIF1α与MVD在上皮性卵巢癌中的表达.docVIP

HIF1α与MVD在上皮性卵巢癌中的表达 【摘要】目的探讨卵巢上皮性肿瘤中缺氧诱导因子1α（HIF1α）与微血管密度（MVD）的关系。方法采用原位杂交和免疫组化法检测66例卵巢上皮性肿瘤和正常卵巢组织中HIF1α mRNA和蛋白的表达水平，用 CD105标记MVD。结果① HIF1α mRNA及HIF1α在卵巢上皮癌和交界性肿瘤中的表达高于良性肿瘤和正常卵巢组织(Ｐ0.05)。CD105MVD值呈卵巢上皮癌＞交界性肿瘤＞良性肿瘤(Ｐ0.05)。 ② HIF1α和CD105MVD值明显正相关，且组织分化程度越低，表达越高(Ｐ0.05)。结论HIF1α对卵巢癌的发生、发展具有重要作用。 【关键词】 卵巢上皮性肿瘤;HIF1α;MVD;CD105 The Chinical Significance of HIF1alpha; and MVD Expression in Epithelial Ovarian Carcinoma CHEN Suqin, GAO Liya, ZHANG Yinghui Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000,ChinaAbstract：Objective To study the relationship between the expression of hypoxia inducible factor1alpha;（HIF1alpha;）and microvessel density （MVD） in epithelial ovarian tumors.Methods The 66 specimens of epithelial ovarian tumor and normal ovarian tissue samples were studied by SP immunohistochemical techniques and in situ hybridization techniques to explore the expression of HIF1alpha; mRNA and protein, and the microvessel density marked by CD105. Results ① The expression level of HIF1alpha; and HIF1alpha; mRNA in borderline and malignant ovarian tumors were higher than benign ovarian tumors and normal ovarian tissues(Ｐ0.05).CD105MVD is higher in malignant ovarian tumors than borderline and benign ovarian tumors(Ｐ0.05).② The correlation of HIF1alpha; and CD105MVD was positive, and were negative with histological differentiation(Ｐ0.05).Conclusion HIF1alpha; may play an important role in occurrence and development of epithelial ovarian tumors. Key words：Epithelial ovarian tumor;HIF1alpha;;MVD;CD105 实体肿瘤的生长和转移依赖于肿瘤细胞对缺氧的适应和新生血管形成。缺氧诱导因子1alpha;（hypoxia inducible factor1alpha;，HIF1alpha;）是在缺氧条件下对基因发挥调控作用的一种转录因子，可通过上调VEGF的表达促进肿瘤血管形成，但HIF1alpha;与肿瘤微血管密度（MVD）的相关性尚无定论。CD105是一种新生血管的特异标志物，优先表达于增生状态肿瘤组织血管内皮细胞上，对微血管内皮细胞染色具有高度敏感性。但CD105在卵巢肿瘤方面的报道国内外甚少见，因此本研究采用原位杂交和免疫组化（SP法）研究在卵巢上皮性肿瘤中HIF1alpha;与CD105所标记的MVD的关系。 1 材料与方法 1.1 材料 选自2002年3月～2004年7月间我院手术切除并经病理证实的卵巢上皮性肿瘤和正常卵巢组织标本共66例：(1)卵巢上皮癌27例