p53、p21WAFCIP1和MDM2在子宫颈鳞状细胞癌中的表达及意义.docVIP

p53、p21WAF/CIP1和MDM2在子宫颈鳞状细胞癌中的表达及意义 【关键词】 p21WAF/CIP1；MDM2；p53；子宫颈癌 摘要：目的: 探讨p53、p21WAF/CIP1和MDM2蛋白在子宫颈鳞癌中的表达及其相互关系。方法: 采用免疫组织化学方法研究在43例子宫颈癌、20例子宫颈上皮内瘤变（CIN）、15例正常组织中p53、p21WAF/CIP1及MDM2的表达情况。结果: p53、p21WAF/CIP1及MDM2在43例子宫颈癌中阳性率分别为34.88％、30.23％、25.58％，在20例CIN中的阳性率分别为5％、60％、15％，在正常组织中未见阳性表达, p53、p21WAF/CIP1在3种不同组织间的表达差异有统计学意义（P＜0.05），MDM2在3种不同组织间的表达差异无统计学意义(P0.05)。p21WAF/CIP1蛋白表达与p53有关（P＜0.05），MDM2蛋白表达与p53无关（P＞0.05）。结论: p21WAF/CIP1蛋白表达与p53有关，是子宫颈癌发生的早期事件，MDM2蛋白表达与p53无关。 关键词：p21WAF/CIP1；MDM2；p53；子宫颈癌 Expression and significance of p53, p21WAF/CIP1 and MDM2 in cervical squamous cell carcinoma SHI Yonghua, Lalai?Suzuke (Department of Pathology, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830054, China) Abstract: Objective: To determine expression of p53, p21WAF/CIP1 and MDM2 in cervical squamous cell carcinoma and relation between them. Methods: Immunohistochemistry was used to detect p53, p21WAF/CIP1 and MDM2 proteins in 43 cases of cervical cancer, 20 cases of CIN and 15 cases of normal group. Result: The positive rates of p53, p21WAF/CIP1 and MDM2 in 43 cases of cervical cancer were 34.88%, 30.23%, 25.58%. The positive rates of p53, p21WAF/CIP1 and MDM2 in 20 cases of CIN were 5%, 60%,15%, respectively. p53, p21WAF/CIP1 and MDM2 proteins were not detected in 15 cases of normal group. The differences of p53 and p21WAF/CIP1 among the there groups were significant (P0.05). The difference of MDM2 expression among the there groups was not significant (P0.05). Expression of p21WAF/CIP1 was correlated with p53 (P0.05). Expression of MDM2 was not correlated with p53 (P0.05). Conclusion: The expression of p21WAF/CIP1 was related to p53, p21WAF/CIP1 was an early event in the carcinogenesis of uterine cervix. The expression of MDM2 is not related to p53. Key words: p21WAF/CIP1; MDM2; p53; cervical cancer 子宫颈癌是女性最常见的恶性肿瘤，占女性恶性肿瘤死因第二位［1］。p53基因是一重要的抑癌基因，参与DNA损伤修复、细胞凋亡等过程,其功能丧失与子宫颈癌的发生有关。p53基因可诱导其下游靶基因p21WAF/CIP1、MDM2表达。本研究采用免疫组化二步法探讨p53 、p21WAF/CIP1及MDM2在子宫