MMP-3TIMP-3表达失衡在子宫内膜异位症中的临床意义.docVIP

MMP-3/TIMP-3表达失衡在子宫内膜异位症中的临床意义 【摘要】目的： 探讨基质金属蛋白酶-3（MMP-3）及其组织抑制剂（TIMP-3）在子宫内膜异位症发生、发展中的作用。方法： 采用免疫组织化学方法（SABC法）检测30例异位子宫内膜组织和30例正常子宫内膜组织（对照组）中MMP-3，TIMP-3的表达情况。结果： 子宫内膜异位症组异位内膜腺体和间质中MMP-3蛋白表达大于正常对照组，差异有统计学意义（P0.01）。子宫内膜异位症组异位内膜腺体和间质中TIMP-3蛋白表达弱于正常对照组子宫内膜腺体中TIMP-3蛋白表达,差异有统计学意义；MMP-3随子宫内膜异位症临床分期增高表达上调，TIMP-3则随临床分期增高表达下调。结论： MMP-3/TIMP-3表达失衡在子宫内膜异位症发生发展过程中可能有着重要作用。 【关键词】 子宫内膜异位症；MMP-3/TIMP-3； 免疫组织化学 Clinical significance of imbalanced expression ofMMP-3/TIMP-3 in endometriosis HE Yue1, KONG Li-na2 (1. Department of Obstetrics and Gynecology, the Affiliated Fourth Hospital of Jiangsu University，Zhenjiang Jiangsu 212001, 2. Department of Obstetrics and Gynecology, the Affiliated Yijishan Hospital of Wannan Medical College， Wuhu Anhui 241001,China) ［Abstract］ Objective: To investigate the role of matrix metalloproteinase-3（MMP-3）and tissue inhibitor of metalloproteinase-3 （TIMP-3）in the pathogenesis of endometriosis(EMs ). Methods： Immunohistochemical method(SABC) was employed to detect the expression of MMP-3 and TIMP-3 in ectopic endometrial tissues from women with endometriosis(30 cases) and the normal controls(30 cases). Results： MMP-3 protein expression level in ectopic endometrial gland and stroma were significantly higher than that in normal endometrium( the value of P less than 0.01), while the protein expression level of TIMP-3 was significantly lower than that in normal endometrium ; the expression of MMP-3 was up-regulated with the increasing clinical stage of endometriosis, but the expression of TIMP-3 was down-regulated with the increasing clinical stage. Conclusion： The imbalanced expression of MMP-3 / TIMP-3 might play an important role in the pathogenesis and development of endometriosis. ［Key words］ endometriosis; MMP-3/TIMP-3; immunohistochemical method 子宫内膜异位症（endometriosis，EMs）的发病原因及发病机理至今尚未阐明，已有不少学者研究发现早期的异位内膜细胞通过粘附性糖蛋白分子粘附于腹膜间皮表面，继而侵入细胞外基质(extracellertrix，ECM)并种植于腹膜，基质金属蛋白酶(matrix metalloproteinases，MMPs)是破坏和降解ECM的重要酶类，金属蛋白酶组织抑制剂(tissue inhibi