MMP-9和TIMP-1在不明原因不孕者子宫内膜的表达.docVIP

MMP-9和TIMP-1在不明原因不孕者子宫内膜的表达 【摘要】 目的检测基质金属蛋白酶-9(MMP-9)和组织金属蛋白酶抑制物-1(TIMP-1)在不明原因不孕、正常早孕蜕膜组织和正常子宫内膜中的表达，探讨其与不明原因不孕关系。方法应用免疫组化S-P法，检测MMP-9和TIMP-1在30例不明原因不孕症患者的种植窗期子宫内膜、20例早孕蜕膜组织和20例正常子宫内膜中的表达情况。结果MMP-9和TIMP-1在宫内早孕蜕膜组织表达程度最高，在正常子宫内膜中表达较高，而在不明原因不孕子宫内膜表达水平显著降低，MMP-9和TIMP-1在三组之间比较,差异均有统计学意义(P均0.05)。结论MMP-9和TIMP-1在不明原因不孕子宫内膜中呈低水平表达，且无分泌期表达水平的增强，这可能会导致子宫内膜接受性的降低，使着床失败引起不孕。 【关键词】 不孕症；基质金属蛋白酶；基质金属蛋白酶抑制物；子宫内膜；蜕膜 The expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 protein in cycling endometrium of patients with primary unexplained infertility. LI Bin, DU Xing-gui, YAO Jun-xia, et al. Department of Pathology, Yunyang Medical College, Shiyan 442000, Hubei, P. R. China Abstract：Objective To detect the expression of matrixmetalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 protein(TIMP-1) in cycling endometrium of patients with unexplained infertility, normal endometrium and normal early pregnant deciduas and explore the relationship between the expression of MMP-9 and TIMP-1 in unexplained infertility and normal pregancy. Methods Immunohistochememstry(S-P technique) and pathologic image semi-quantification were used to detect the expression of MMP-9 and TIMP-1 in endometrium of 30 unexplained infertility patients and 20 normal early pregnant deciduas and 20 normal endometrium. Results The expression of MMP-9 and TIMP-1 in normal early pregnant deciduas was the highest,showing significant difference as compared with other two groups(P0.05) The expression of MMP-9 and TIMP-1 was also high in normal endometrium as compared with other two groups(P0.05). But the expression of MMP-9 and TIMP-1 in endometrium of patients with unexplained infertility was significantly reduced as compared with other two groups(P0.05). Conclusion In unexplained infertility women, the low expression of MMP-9 and TIM P-1 protein in the implantation window phase may cause the failure of implantation and results in the infertility. Key words：Unexplained i