急性脊髓损伤后perk pcna gfap的表达和意义以及u0126对其表达的影响-expression and significance of pert pcna gfap after acute spinal cord injury and the influence of u 0126 on its expression.docx

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急性脊髓损伤后perk pcna gfap的表达和意义以及u0126对其表达的影响-expression and significance of pert pcna gfap after acute spinal cord injury and the influence of u 0126 on its expression.docx

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急性脊髓损伤后perk pcna gfap的表达和意义以及u0126对其表达的影响-expression and significance of pert pcna gfap after acute spinal cord injury and the influence of u 0126 on its expression

cord.ComparedwithshaminjurygrouptheexpressionofpERKandPCNAininjuredspinalcordexistedsignificantdifference(P0.05).1dayafterSCItheirexpressionincreasedprominently,andpeakedat7thday,andattheendof14thdaybegandrop.3.InshaminjurygroupGFAPexitedincytoplasmofastrocytes.ComparedwithshaminjurygrouptheexpressionofGFAPininjuredspinalcordexistedsignificantdifference(P0.05).itsexpressionwasincreasedprogressivelyform1stto14thdayafterSCI.4.TheexpressionofpERKandPCNA;PCNAandGFAP;pERKandGFAPform1stto14thdayafterSCIallhadpositivecorrelation(r1=0.907，P0.05；r2=0.823，P0.05；r3=0.807，P0.05).5.TheexpressionofpERK,PCNAandGFAPbetweensimpledamagegroupandDMSOgroupdidn’texistedsignificantdifference（P0.05）.ComparedwithsimpledamagegroupandDMSOgrouptheexpressionofpERK,PCNAandGFAPinU0126treatmentgroupat7thdayand14thdaywasdown-regulated,andexistedsignificantdifference(P0.05).Conclusion1.TheexpressionofpERK,PCNAandGFAPincreasedprominentlyafterSCI,andhadpositivecorrelationwitheachother.thoseexperimentalresultssuggestedthatMEK/ERKsignalpathwaymaybeactivatedafterSCIwhichcanincreasethephosphorylationofERK,andfurtherpromoteastrocyteintocellcycle,highertheproliferationofastrocyte,whichleadtotheformationoftheglialscar.2.MEKinhibitorsU0126canpromoteratsacutespinalcordinjuryratsafterdoublehindethologyrecovery.3.TheinhibitorsofMEKU0126cansuppresstheproliferationofastrocyteandreducetheformationoftheglialscar.ItsmechanismmightreducethephosphorylationofERKinthespinalcordinjurysitebyMEK/ERKsignalpathway,andfurthersuppressastrocyteintocellcycle,lowertheproliferationofastrocyteinordertoprovidefavorableconditionsforrecoveryofneurologicalfunction.Keywordsspinalcordinjury;glialscar;U0126;phosphorylatedextracellularsignal-regulatedkinase;proliferatingcellnuclearantigen;glialfibrillaryacidicprotein英文缩略词表英文缩写英文全称中文全称Astastrocyte星形胶质细胞BDAbiotindextranamine生物素化萄聚糖胺BBBBasso,Beattie,Bresnahan运动功能评分LocomotorscoringCNSCentralneuronsystem中枢神经系统CDK4Cyclindependentkinases细胞周期蛋白依赖激酶dday天DMSOdimethylsulfoxide二甲基亚砜DABdiaminobenzidine二