几株微囊藻毒素降解菌和溶藻菌分离鉴定及作用效果-isolation, identification and effect of several microcystin - degrading and algicidal bacteria.docx

英文摘要几株微囊藻毒素降解菌和溶藻菌的分离鉴定及作用效果Isolation and Identification of Several Strains of Microcystins Degrading and Algicidal Bacteria and Research on Their Biodegrading and Algicidal Effects AbstractThree strains of bacteria were isolated from different eutrophic water bodies. They were numbered G-2, A-8 and L-9 by our lab. In order to ensure their taxonomic status, the morphological, physiological characteristics and Phylogenetic analysis based on 16S rDNA sequence were used to identify three strains of bacteria. According to the results, the three strains G-2, A-8 and L-9 were respectively identified as Bacillus cereus, Acinetobacter Haemolytius, Leucobacter tardus.The microcystins degradation efficiency of A-8 and L-9 were measured by M9 medium (added MC). According to ELISA testing, the strains A-8, L-9 had good biodegradation of microcystins and the removal rates were 30.4% and 41.9% respectively on the third day.The rules of growth and algicidal effects of G-2 and A-8 were also studied, and their algicidal mechanisms were discussed initially.These results indicated that the strains algicidal bacteria removed algae by their metabolite substances, and the metabolite substances secreted by G-2 and A-8 could remain active at high temperature. The initial algal concentrations influence the removal rates of G-2 and A-8 strongly. For G-2 the greater the initial algal concentration the faster the degradation of algal, but the influence of initial algal concentration was weak on algicidal efficiency of A-8. The temperature, illumination and pH also had influence on the algicidal effects. Accordingto the results, the removal rates of G-2 and A-8 were the best at 30 ℃ or 35 ℃. The A-8strain showed the best algicidal effects when were under pH arrange from 7.02 to 9.97, while the G-2 was pH 9.97. The algicidal effects of two strains under different illumination were tested. And the results indicated that the longer of the illumination the几株微囊藻毒素降解菌和溶藻菌的分离鉴定及作用效果英文摘要faster the degr