(食品质量和 与安全研讨会)Cardiac Calcium Channel Expression in Heart Specific GH Receptor Gene Disrupted Mice.pptx

Cardiac Calcium Channel Expression in Heart Specific GH Receptor Gene Disrupted MiceXingbo LiuCalciumThe reason I choose Micronutrient. 1 kg in adult body99% in skeleton as calcium phosphate salts.in ECFFunction:Structural functionSignaling functionEnzymatic functionHypothalamusGHRHSSTAnteriorPituitaryGH FatLiverBoneIGF-1 MuscleKidney HeartGH in HeartMaintain muscle mass and strengthen cardiac structure (Castellano, 2009)Increase LV mass (amino acid uptake, actin gene expression) Decrease wall stress and vascular resistance (NO system)Increase contractility (Myosin-actin phenotype, calcium channel sensitivity) Increase cardiac performanceGH resistantRole of Ca++ in CardiomyocytesCa++Na+NCX1Ca++Sarcoplasmic ReticulumL-type channelRyanodine ReceptorL-type Calcium ChannelSERCACa++T-TubuleT-tubuleCa++Ca++ContractionCa++iC-GHRKO TimelineN=5TAMMerCreMer/FloxedgroupN=14N=9OilTAMFloxedgroupN=9N=18OilN=9Age (weeks)041617BirthWeanTAM/OilInjectionDissectTissuesSpecific Disruption of the GHR Gene in the HeartGHR mRNA expression 80% reducedGH induced STAT5 phosphorylation disruptedCardiac Calcium Channel mRNA Expression in iC-GHRKO***P0.05ConclusionCreated an inducible cardiac specific GHR disrupted mouseLoss of GHR specifically in cardiomyocytes decreases the mRNA expression of SERCA2a increases the expression of NCX1similar to the calcium channel expression observed in heart failure (Nattel, 2007) Future studies in iC-GHRKO mouseexamine calcium channel expression at the protein level assess calcium channel function—Ca++ peak, Ca++ influxcompare calcium channel expression to global GHR KO mouseThank you~ECF: extracellular fluid9mmol in serumStructural function: Supporting material in bones. Present as calcium phosphate.Signalling function: Intracellular calcium functions as a second messenger for some hormones.Enzymatic function: Calcium acts as a coenzyme for clotting factors.Calcium also causes the release of Acetylcholine from Pre-synaptic terminal in the transmission of