(食品质量和 与安全研讨会)Isolation and identification of osmotolerant yeasts from quick-freeze Kiwi fruit processing.pptx

Isolation and identification of osmotolerant yeasts from quick-freeze Kiwi fruit processingStudent: Niu ChenGuider: Prof. Yue Tianli Contents1. Background2. Material and methods3. Results4. Discussion5. Conclusion1.BackgroundKiwi fruitConsequenceDefinitionYeasts tolerating 50% (w/w) glucose concentration were termed osmotolerant yeasts.Blown cans / packages, rupture of packages, food spoilage, deterioration of food quality, economic loss, taint brand imageKiwi fruit production in Shaanxi accounts for 56% national production in 2012.Processing is growing.Kiwi juice, quick-freeze, dried kiwi.Aim: To determine main osmotolerant yeasts that spoil quick-freeze kiwi products. 2. Material and methods-sampling schemeBefore processingDisinfectionQuick-freezeWashingpeel, pulp, air, slicesliceslice, water, air quick-freeze kiwi fruit processing lineslice, air2. Material and Methods-isolation and identificationDistribution of osmotolerant yeasts over processing segmentsFig.2 The distribution of osmotolerant yeasts on the processing procedures of quick-freeze kiwi fruitGlucose toleranceFig.5 The growth curve of omsotolerant yeasts in 60% (w/w) glucose concentrationFig.3 The growth curve of omsotolerant yeasts in 2% (w/w) glucose concentrationFig.4 The growth curve of omsotolerant yeasts in 50% (w/w) glucose concentrationGas productionFig.6 The gas production of osmotolerant yeasts (P 0.05)4. DiscussionHygiene indicators H. uvarum, D.hansenii, L. elongisporusNot thorough disinfection yeasts floating in airHighly osmolerant species and gas-forming species D.hansenii, L. elongisporus; H.opuntiae, M.pulcherrimaFrequent occurring species sporadic occurring species H. uvarum, C. tropicalis; M. pulcherrima, L. elongisporus125.ConclusionIdentification of osmotolerant yeasts on the processing line of quick-freeze kiwi fruit for the first time.To prevent spoilage accident, disinfection measures should be taken aiming at both frequent occurring species and sporadic ones.Than