il13对人支气管上皮细胞粘蛋白1表达的影响-effect of il13 on expression of mucin 1 in human bronchial epithelial cells.docx

独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的 研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个 人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体， 均已在文中以明确方式标明。本人完全意识到本声明的法律结果由本人承担。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保 留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本 人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索， 可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□，在年解密后适用本授权书。 不保密□。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录中英文对照词汇表····················································································· 1中文摘要··································································································2Abstract 3前言·································································································· 5材料与方法···························································································· 7实验结果····························································································· 17讨论·································································································21References23综 述··································································································· 27 附录：硕士攻读期间研究成果·····································································44 致 谢··································································································· 45中英文对照词汇表英文缩写HBEc英文全称Human Bronchial Epithelial Cells中文全称人支气管上皮细胞Th2 TLR MUC1 mRNA DC DEPC DMSO BSA FBS PBS SDSSDST-helper type-2 Lymphocytes Toll Like ReceptorMucin 1Messenger ribonucleic acid Dendritic CellsDiethyl Pyrocarbunate Dimethyl Sulfoxide Bovine Serum Albumin Fetal Bovine Serum Phosphate Buffered Salin Sodium Dodecyl SulfateSodium Dodecyl Sulfate辅助性 2 型 T 淋巴细胞Toll 样受体 粘蛋白 1 信使核糖核酸 树突状细胞 焦碳酸二乙酸 二甲基亚砜 牛血清白蛋白 胎牛血清 磷酸盐缓冲液 十二烷基硫酸钠聚丙烯酰胺凝胶电泳cDNAPolyacrylamide GelComplementary Deoxynucleic Acid互补脱氧核糖核酸OD PVDF EDTAECLOptical Density Polyvinylidene FluorideEthylene diaminete traacetic acidElectrochemiluminescence光密度聚偏氟乙烯 乙二胺四乙酸 电化学发光IL-13 对人支气管上皮细胞粘蛋白 1 表达的影响硕士研究生： 孟 丹导师： 许淑云 教授 华中科技大学同济医学院附