抗瘢痕成纤维细胞增殖有效成分的筛选及其作用机理分析-screening of effective components against proliferation of scar fibroblasts and analysis of their mechanism of action.docxVIP
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抗瘢痕成纤维细胞增殖有效成分的筛选及其作用机理分析-screening of effective components against proliferation of scar fibroblasts and analysis of their mechanism of action
1.5 小结................................................................................................................................ 29 第二章 三七对瘢痕成纤维细胞的增殖抑制作用研究 ........................................................ 30 2.1 实验材料........................................................................................................................ 302.1.1 实验细胞 ................................................................................................................. 302.1.2 实验试剂 ................................................................................................................. 302.1.3 实验仪器 ................................................................................................................. 312.2 实验方法........................................................................................................................ 312.2.1 三七的分段提取 ..................................................................................................... 312.2.2 成纤维细胞抑制增殖率的检测 ............................................................................. 322.2.3 乳酸脱氢酶(LDH)的测定 322.2.4 流式细胞仪法分析细胞周期 ................................................................................. 322.2.5 抗成纤维细胞增殖药效成分的有效成分分析及鉴定 ......................................... 332.2.6 有效成分抗成纤维细胞增殖的验证 ..................................................................... 342.2.7 活性成分的反向对接与靶点预测 ......................................................................... 342.2.8 统计学分析 ............................................................................................................. 352.3 实验结果........................................................................................................................ 352.3.1 三七各提取物抗瘢痕成纤维细胞增殖作用的考察 ............................................. 352.3.2 三七乙酸乙酯提取物对细胞周期的影响 ............................................................. 352.3.3 抗成纤维细胞增殖成分的谱效分析及鉴定 ......................................................... 362.3.4 人参皂苷 Rh1 和人参皂苷 Rg1
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