宽礁膜monostroma latissium多糖的分离 结构和抗凝血活性研究-study on isolation structure and anticoagulant activity of mono stroma latissimum polysaccharide from wide reef membrane.docxVIP

Studies on Isolation, Structure and Anticoagulant Activity of the Sulfated Polysaccharide from Monostroma latissimum AbstractMonostroma latissium is a solt of green algae plant in ocean. It grows widely inthe brackish water area in the upper part of the intertidal zone. Monostroma latissium exhibits many biological activities such as anticoagulant, antiviral, antioxidant activities and radioprotective effect. It has been confirmed that the main active substance in Monostroma latissium is soluble sulfated polysaccharide.In this paper, the polysaccharide from Monostroma latissium was extracted by cool water, boiled water and alkali liquid successively. Three kinds of polysaccharides were obtained and named as CE, HE and AE, respectively. The chemical characteristics of the polysaccharides were analyzed by chemical method, GC and HPLC. The results showed that the total yield of the polysaccharides was 40.41% and the three polysaccharides were soluble sulfated polysaccharides with the different sulfate content. The sulfate contents of polysaccharides are 21.33%, 24.92% and 25.34%, respectively. Three sulfated polysaccharides were found to contain mainly rhamnose with small amounts of glucose and xylose and trace amounts of glactose and mannose. The uronic acid is consisted of glucuronic in the sulfated polysaccharide.Based on the physicochemical analysis, three pure fractions HEA, HEB and HEC were obtained from HE using ion-exchange chromatography and low-pressure gel permeation chromatography. The chemical characteristics and primary structures of the pure fractions were investigated. The result showed that HEA was a polysaccharide with high molecular size (725.4kD). The molecular weights of HEB and HEC (54.8 kD, 68.8 kD) are similar. The monosaccharide compositions of the three polysaccharides are similar. The backbone of HEA and HEB were consisted of 1,3-Rha, 1,2-Rha and the sulfate groups were substituted at C-4 or at C-3 in 1,2-Rha.While HEB was a kind of sulfated