利用选择导入系进行水稻耐低磷鉴定与qtl定位研究-study on identification of low phosphorus tolerance and qtl mapping of rice by using selective introgression lines.docxVIP

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利用选择导入系进行水稻耐低磷鉴定与qtl定位研究-study on identification of low phosphorus tolerance and qtl mapping of rice by using selective introgression lines.docx

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利用选择导入系进行水稻耐低磷鉴定与qtl定位研究-study on identification of low phosphorus tolerance and qtl mapping of rice by using selective introgression lines

RM481、RM402 是进一步改良轮回亲本蜀恢 527 耐低磷性的重要位点。RM335、RM5463、RM219、RM254、RM341、RM571、RM316 和 RM224 这 8 个位点是蜀恢527 在低磷环境下稳产的重要区段。RM200、RM6959、RM108 是进一步改良轮回亲本明恢 86 耐低磷性的重要位点。 RM335、RM169、RM254 和 RM5349 这 4 个位点是明恢 86 在低磷环境下稳产的重要区段。推测这些 QTL 可作为水稻耐低磷产量性状分子育种的重要候选区域。本研究通过大田全生育期筛选和水培的苗期交叉筛选，获得了一批优良导入系，这些材料既可以作为培育磷高效利用水稻品种的育种材料，又可以用于耐低磷的遗传机制剖析。关键词：水稻，导入系，耐低磷，产量，QTLIIAbstractIn this study, Shuhui527 and Minghui86, the two widely used elite indica restorers in China, were used as recurrent parents, two indica varieties Yetuozai and 2004 as donor for developing four backcrossing introgression line (IL) populations. The bulks of four BC2F2 backcrossing populations were imposed critical screening for low phosphorus tolerance (LPT) under the red soil condition with phosphorus deficiency in Xuancheng, Anhui province. The progeny of the BC2F4 populations and their parents were tested for comprehensive phenotypic evaluation in the whole growth stage at the field condition .Meanwhile, the two IL populations were investigated at seedling stage using nutrient solution in the glasshouse.Compared with the control condition, B treatment (normally fertilizing under barren soil) caused the significant reduction of plant height and spikelet number per panicle of the recurrent parent Shuhui527 and Minghui86, but no significant changes were observed in panicle number per plant, spikelet fertility and thousand grain weight, resulting in the yield loss of Shuhui527 and Minghui 86 under B treatment. Under P treatment (phosphorus stress under barren soil), plant height, filled-grain number per panicle, thousand grain weight, grain yield per plant and harvest index of Shuhui527 and Minghui86 were significant affected, while panicle number per plant and spikelet fertility were not obviously affected, indicating the reduction of filled-grain number per panicle and thousand grain weight were the main reason for the changing of grain yield of Shuhui527 and Minghui86. Transgressive variations were