利用重组自交系群体创建抗倒高产高丹草新种质分析-analysis of establishing new germplasm of anti-lodging and high-yielding sorghum sudanense by recombinant inbred line population.docxVIP

Using Recombinant Rnbred Rines Rreate Rodging Rielding Sorghum Sudan the Grass Germplasm Research Abstract Lodging is an important factor that affect the yield of Sorghum Hybrid Sudangrass. To further cultivate high yield, new lodging resistant varieties of Sorghum Hybrid Sudangrass, we first created the F9 recombinant inbred lines 118 of Sorghum Hybrid Sudangrass(Sorghum 314A × brown shell sudangrass). Then we measured plant height, ear length, stem fresh weight, stem weight, section II, 1/2 the height of the bending force, the center of gravity height, root weight and other agronomic traits and calculated the coefficient of its lodging. The correlation of the lodging coefficient and main agronomic traits and the SRAP molecular markers were analysised. The main results are summarized as follows: We created the F9 recombinant inbred lines of the Sorghum Hybrid Sudangrass based on descendant materials of 314A× brown shell sudangrass. We screened three (11gz-12 11gz-95 and 11gz-114), strong lodging resistance, high yield materials in 118 F9 Sorghum recombinant inbred lines . We found that the bigger the lodging coefficient the greater Lodging area of the Sorghum Hybrid Sudangrass .Meanwhile, lodging coefficient was highly positive correlation with plant height, stem fresh weight and stem weight ,It was negative correlation with the root weight and breaking-resistant strength. The result indicate that lodging coefficient evaluation is feasible to evaluate lodging resistance of Sorghum Hybrid Sudangrass . Through optimization of sudangrass SRAP-PCR reaction system factors to establish theoptimal reaction Concentration or content of the total systemy. The 20μl total volume components are as follows: 10*Buffer 2.5ul, MgCl2 2.5 μl, DNTP 2.5ul, primer 2μl Tap enzyme 0.15μl, template DNA 1.0μl, ddH2O 9.35μ. Research found that the results of analysis of agronomic characters are consistent with molecular marker analysis . We screened strong lodging resistance, high yield mate