内皮抑素对视网膜新生血管中.DOC

内皮抑素抑制小鼠视网膜新生血管中 整合素β3表达的实验研究 辛梅 张美霞 张军军 严密 夏庆杰 曹桂群 [摘要] 目的 观察血管生成抑制因子内皮抑素（endostatin, ES）β3（Integrinβ3）表达的影响。方法　通过缺氧的方法建立小鼠视网膜新生血管模型36只，随机分为给氧组及ES治疗组，每组各18只小鼠。ES治疗组小鼠在出氧箱后12h、36h，玻璃体腔内注射1μgμl的 ES1μl。另有18只同龄小鼠生活在正常氧环境中作为正常对照。提取三组小鼠视网膜总RNA，通过RT-PCR方法定量检测Integrinβ3在三组小鼠视网膜中的表达。结果 RT-PCR结果显示氧致视网膜病变视网膜中Integrinβ3的表达，与正常对照组比较有统计学意义（P0.05）；ES作用后Integrinβ3的表达减少（P0.05）。 ES抑制视网膜新生血管生成可能是通过抑制Integrinβ3 mRNA基因β3；荧光定量聚合酶链反应 Effects of endostatin on the expression of Integrinβ3 in the retina of oxygen-induced retinal neovascularization. XIN Mei, ZHANG Meixia, ZHANG Junjun, YAN Mi, XIA Qingjie, CAO Guiqun. The West China Ophthalmic Center, Sichuan University, Chengdu, 610041, China. Correspongding author: ZHANG Meixia, Email: zhangmeixia@ [Abstract] Objective To investigate the effect of endostatin on the expression of Integrinβ3 in the retina of oxygen-induced retinal neovascularization. Methods Thirty-six one-week-old C57BL/6J mice were established the oxygen-induced retinal neovascularization model. In treatment group, 1μl endostatin(1μg/μl) were injected into the vitreous after 12h, 36h out of the oxygen box. Eighteen mice were cultured in the room air to make the control group. Total RNA were extracted from retinas of 基金项目：国家自然科学基金教育部重点项目（108178） 作者单位：610041 成都, 四川大学华西医院眼科 通讯作者：张美霞，Email: zhangmeixia@ three mice groups, the quantity of intergrinβ3 mRNA in retinas was analyzed with quantitative real-time PCR with the special primers. Results Quantitative real-time PCR results indicated that in the retinal neovascularization, the expression of intergrinβ3 is up-regulated, in the treatment group the expression of VEGF is down-regulated by ES. Conclusions Endostatin inhibits the oxygen-induced intravitreal retinal angiogenesis by down-regulated intergrinβ3 which related to the angiogenesis. [Key words] Retinal neovascularization; Angiogenesis inhibitor; Endostatin; Animal disease models; In