两种纳米与常规材料dna损伤和亚慢性毒性的比较分析-comparative analysis of dna damage and subchronic toxicity between two nano-materials and conventional materials.docxVIP

第二军医大学硕士学位论文 moment and comet tail length compared to control in the Comet assay after an exposure of 6 h; micro-TiO2 did not induce the tail DNA content, Olive tail moment, comet tail length changed. Our data demonstrates that nano-TiO2 particles possess a toxic potential in A549 cells which may be significantly higher than micro- ones; the toxic effects occurred earlier for time and lower for dose compared with micro-TiO2, and nano-TiO2 particles can induce DNA damage, but micro- one did not. In conclution, the nano-and micro-TiO2 may be with different toxicity and toxic properties. 3、Comparative study on sub-chronic toxicity in rats caused by nano- and micro- ZnO. SPF SD rats were used as the tested animals, they were randomly divided into 5 groups, n=8. Once a day for 42 days by intraperitoneal injection of the same dose(10mg/mL) of different scale nano-ZnO（30nm、50nm、100nm）and micro-ZnO(≤1μm),compared sub-chronic .The results showed that 30nm ZnO can lead to liver, sleep, kidney, bone marrow and testicular function or organizational changed, and other nano-ZnO without changes in renal function; micro-ZnO can cause lung and kidney coefficient increased, but has not seen functional or organizational change. Nano- and micro-ZnO can cause bone marrow blood cells proliferative anemia active, leading to increase the number of neutrophisl, but the relative proportion of lymphocytes and basophilic cells was decreased, which 30nm and 50nm were the most obvious. Our data demonstrates that the sub-chronic toxicity of nano-ZnO was significantly higher than the micro-one; the sensitivity target organs of nano-ZnO are the testes, liver, bone marrow and kidney;and with the scale reduced, the number of affected organs and the level of damage increased. The target organ of micro-ZnO is the bone marrow, but liver, kidney, lung are possible. 4、Comparative study on sub-chronic toxicity in rats caused by nano- and micro-TiO2. Used the same animals and test methods to the first par