两种荛花属药用植物化学成分和生物活性分析-analysis of chemical constituents and biological activities of two medicinal plants in the genus juhua.docx

(6),methylarctigenin(7),(+)-isolariciresinol(8),(-)-isolariciresinol(9),1H,3H-furo[3,4-c]furan-1-ol,tetrahydro-4-(4-hydroxy-3-methoxy-phenyl)-,(1R,3aS,4R,6aS)-rel-(10),(+)-epipioresinol(11),(-)-lariciresinol(12),(+)-lariciresinol(13),(-)-dehydrodiconiferylalcohol(14),(+)-pinoresinol(15),4-hydroxykobusin(16),Hprostratin(17),prostratinQ(18),wikstrotoxinD(19),pimelotidesA(20)andC(21),pimeleafactorsS7(22),S6(23),P2(24),和P3(25),wikstroelidesR(26),S(27),T(28),U(29),和Q(30),1-propanone,3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-(31),phenethylalcohol(32),trans-p-hydroxycinnamicacid(33),(-)-matairesinol4-O-glucoside(34),(+)-pinoresinol-β-D-glucoside(35),corchoionosideC(36),2-Cyclohexen-1-one,4-[(3S)-3-(β-D-glucopyranosyloxy)butyl]-4-hydroxy-3,5,5-trimethyl-,(4S)-(37),其中化合物5，18，26，27，28，29，30是新化合物，化合物10是新天然产物。除化合物19，其余36个化合物均为首次从该植物中分离得到。从小黄构中分离得到的化合物分别为β-sitosterol(38),ursolicacid(39),oleanolicacid(40),genkwanin(41),salipurpol(42),sikokianinA(43),sikokianinB(44),wikstrolB(45),wikstrolA(46)，这些化合物均为首次从该植物中分离得到。此外，本文选取人白血病HL-60、肺癌A-549、肝癌SMMC-7721、乳腺癌MCF-7和肠癌SW480五种肿瘤细胞和正常肺上皮细胞BEAS-2B分别对化合物18-30进行了体外细胞毒活性测试。结果显示，化合物26，30仅对HL-60有一定的细胞毒性，对其他四种癌细胞则无细胞毒性；化合物18-25以及27-29对所选的5种人肿瘤细胞株均表现出一定的细胞毒性。对荛花属两种药用植物黄芫花和小黄构化学成分和生物活性的研究有望为我国天然药物资源的利用与开发提供科学依据。关键词：瑞香科；荛花属；二萜；木脂素；双黄酮；细胞毒性AbstractThereareabundantplantresourcesinChina,andrankssecondintheworld.ItisanimportantpathwaytosearchfornovelbioactivenaturalproductsfromtraditionalChinesemedicineandfolkloremedicinalplantsasnewdrugleadingcompounds,anditwillprovidescientificmaterialbasisoftraditionalChinesemedicineandfolkloremedicine.ThegenusofWikstroemia(Thymelaeaceae)iscomposedofabout70species,andwidelydistributedinsoutheastAsia,OceaniaandthePacificIslands.40ofthemareemidictoChina,andmainlygrowninthesouthoftheYangtzeRiver.MostplantsofWikstroemiahavebetterbastfiberwhichwasusuallyusedasrawmaterialsinthemanufactureoffinepaperandrayon.Additionally,manyofthemareornamentalplantforgardencultivation.TheplantsofWikstroemiahaveattractedmuchattentionfrommanyres