两株家蚕病原菌的分类鉴定及其部分特性分析-classification and identification of two silkworm pathogenic bacteria and analysis of their partial characteristics.docxVIP

江苏科技大学农学硕士学位论文rate of STH1 was slower than that of SHB. After 15 days, the conidia output of strain STH1 was 9.82±3.49×108spores/cm2, and SHB was 4.01±2.12×108spores/cm2. The α=0.01 which means there are extremely significant difference between the grower rate two kinds of strains.The ITS gene of strain STH1 and SHB was all 549 bp, and had high similarity with eachother (equal to 100%). Phylogenetic tree showed that STH1 was a member of Beauveria bassiana clade. The complete sequence of 18S rRNA gene of STH1 was 1 766 bp and a insertion sequence of group I introns (1 866 bp) encode homing endonucleases within the gene. The phylogenetic analyse of 18S rRNA, 28S rRNA and IGS gene of STH1 and SHB indicated that they had approximately 100% similarity. So we believed the pathogenic fungi STH1 was from the species Cordyceps bassiana, and it was a novel strain that contains group I introns.Study on the phylogenetic identification and characteritions of strain ST-1 and STH1, could define their taxonomic status, and lay a foundation for the prevention and therapy of diseases caused by ST-1 and STH1.Key words: Pathogenicity, Taxonomic identification, Ribosomal RNA gene, Silkworm (Bombyx mori)江苏科技大学学位论文原创性声明本人郑重声明：所呈交的学位论文，是本人在导师的指导下，独立进 行研究工作所取得的成果。除文中已经注明引用的内容外，本论文不包含 任何其他个人或集体已经发表或撰写过的作品成果。对本文的研究做出重 要贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本声 明的法律结果由本人承担。学位论文作者签名：年月日江苏科技大学学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，同意 学校保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文 被查阅和借阅。本人授权江苏科技大学可以将本学位论文的全部或部分内 容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存 和汇编本学位论文。本学位论文属于：(1)保密□，在年解密后适用本授权书。 (2)不保密□。学位论文作者签名：指导教师签名：年月日年月日第 1 章绪论1.1 研究背景与意义1.1.1 家蚕细菌病蚕病的种类很多，发生原因各异，病征表现也各不相同，通常将蚕病分为传染性 蚕病和非传染性蚕病两种。家蚕细菌病属于传染性蚕病的一种，它是由各种细菌侵染 寄生蚕体引起的蚕病。患细菌病的蚕，尸体失去弹性，很快软化腐烂，故也称之为“软 化病”。这是一种常见的病害，大多是零星分散发病，但不论春蚕、夏秋蚕都有发生 [1]。通常情况下，大量发生的病例比较少见。在生产全局上虽然没有严重威胁，但在 局部地方也有因为储运不当造成的桑叶发酵，或饲养粗放而大量发生。特别是在我国 某些地方推广细菌农药防治农业害虫时，由于忽视安全使用，结果导致大量病原细菌 向蚕区扩散，造成严重损失[2,3]。目前研究表明，对家蚕显示病原性的细菌可分为 3 类：败血细菌、卒倒杆菌及肠道细菌。这 3 类细菌分别引起细菌性败血病、细菌性中毒病及细菌性肠道病[4]。其中 能引起家蚕败血病的细菌种类很多，以能产生卵磷