kisspeptingpr54表达异常与不明原因复发性流产发病关系的研究-study on the relationship between the abnormal expression of kisspeptingpr 54 and the pathogenesis of unexplained recurrent abortion.docxVIP
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kisspeptingpr54表达异常与不明原因复发性流产发病关系的研究-study on the relationship between the abnormal expression of kisspeptingpr 54 and the pathogenesis of unexplained recurrent abortion
Kisspeptin/GPR54表达异常与不明原因复发性流产发病关系的研究英文摘要StudyonrelationshipbetweendysfunctionofKisspeptin/GPR54andthepathogenesisofunexplainedrecurrentspontaneousabortionAbstractObjective:Toinvestigatetheexpressionofkisspeptin/GPR54atmaternal-fetalinterfaceofwomenwithunexplainedrecurrentspontaneousabortion(URSA)andtheprobablepathogenesisofrecurrentspontaneousabortion.Methods:1.Clinicalresearch:Trophoblasticanddecidualtissueswerecollectedfrom32URSAwomenwhomiscarriedageneticallynormalfetusand35womenwhohadvoluntaryabortion.Kisspeptin,GPR54,PRandPIBFwereinvestigatedwithimmunohistochemistry.2.Animalexperiments:(1)Theresorption-pronematingofCBA/J×DBA/2micewereestablishedasthemodelofspontaneousabortionandthenonabortion-pronematingofCBA/J×BALB/cmicewereusedasthemodelofnormalpregnancy.Theplacentatissueswerecollectedonday9andday14ofgestation,thenkisspeptin,GPR54,PRandPIBFwereinvestigatedwithimmunohistochemistry.Theembryoresorptionratewasseparatelycountedonthetwodays.(2)Choosingtheresorption-pronematingofCBA/J×DBA/2mice,whichweredividedequallyintotwogroups,thegroupinjectedwithprogesteroneeverytwodaysaftermatingwastheexperimentalgroup,theotheroneinjectedwithteaoilatthesamedoseonthesamedayswasthecontrolgroup.Theplacentatissueswerecollectedonday9andday14ofgestation,andkisspeptin,GPR54,PRandPIBFwereinvestigatedwithimmunohistochemistry.Theembryoresorptionratewasalsoseparatelycounted.Expressiondifferencesofkisspeptin,GPR54,PRandPIBFwerecomparedbetweenthetwogroupsinordertoobservethechangesofkisspeptin,GPR54,PRandPIBFexpressioninducedbyprogesterone.Results:Kisspeptin,GPR54andPIBFexpressioninsyncytiotrophoblastsweresignificantly英文摘要Kisspeptin/GPR54表达异常与不明原因复发性流产发病关系的研究decreasedinURSAwomenascomparedtocontrols(P0.05).Kisspeptin,PRandPIBFexpressionindeciduasweresignificantlydecreasedinURSAwomenascomparedtocontrols(P0.05).GPR54expressionindeciduasnearlyhadnodifferenceamongstudygroupsandcontrols.PRexpressionwasnegativeindeciduasbothofthetwogroups.Theembryoresorptionrateoftheresorption-pronematingofCBA/J×D
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