肝胆管结石上皮Survivin及PCNA表达与肝胆管癌关系研究.docVIP

肝胆管结石上皮Survivin及PCNA表达与肝胆管癌关系研究 　　[摘要] 目的 探讨肝胆管结石上皮Survivin及增殖细胞核抗原（PCNA）表达与肝胆管癌之间的关系。方法 采集正常对照、肝胆管结石、肝胆管癌患者肝胆管组织，免疫组化法测定肝胆管上皮Survivin及PCNA表达阳性率。结果 正常对照、肝胆管结石、肝胆管癌患者肝胆管上皮Survivin表达阳性率分别为0、42.1%和70.0%。三组间两两比较，差异具有统计学意义（P＜0.05）。正常对照组PCNA阳性率为0，而肝胆管结石、肝胆管癌患者肝胆管上皮PCNA表达阳性率均为100%，强度为++或+++。其中肝胆管结石组的PCNA与病程长短和结石大小呈正相关，肝胆管癌组的Survivin和PCNA表达呈正相关（r＝0.701，P＜0.01）。结论 肝胆管结石上皮组织高表达Survivin及PCNA，提示肝胆管结石上皮有癌变倾向。 　　[关键词] Survivin；PCNA；肝胆管结石；肝胆管癌 　　[中图分类号] R735.8；R657.4 [文献标识码] B [文章编号] 1673-9701（2011）23-53-03 　　 　　The Correlation between the Expression of Survivin and PCNA in Hepatobiliary Epithelium of Hepatolithiasis and Cholangiocarcinoma 　　ZHANG Miaozun1 LI Mingzhu1 GU Xin2 　　1.Department of Hepatobiliary Surgery, the People’s Hospital of Yinzhou, Ningbo 315040, China; 2.Department of Pathology, the People’s Hospital of Yinzhou, Ningbo 315040, China 　　 　　[Abstract] Objective To estimate the correlation between the expression of survivin or PCNA in hepatobiliary epithelium of hepatolithiasis and cholangiocarcinoma. Methods The specimens of hepatobiliary tissue from the paitents of normal control, hepatolithiasis and cholangiocarcinoma were obtained during the surgeries. The expressions of survivin or PCNA were examined by immunohistochemistry. Results The positive expression rates of survivin were 0, 42.1% and 70.0% in control group, hepatolithiasis group and cholangiocarcinoma group respectively. The hepatolithiasis and cholangiocarcinoma groups had statistically higher rates of survivin than that of control group （P＜0.05）. And the rates in cholangiocarcinoma patients were significantly increased, compared to that in hepatolithiasis （P＜0.05）. Hepatobiliary tissue didn’t have PCNA expression in control group. However, the positive PCNA rates in hepatolithiasis and cholangiocarcinoma were both 100% with levels from ++ to +++. PCNA rates in hepatolithiasis were significantly associated with duration and diameter of calculus. A positive correlation was found between surviv