高强度运动对氧化-抗氧化内稳态提升.docVIP

高强度运动对氧化-抗氧化内稳态提升 　　收稿日期：2011-08-25 　　基金项目：广东省科技厅科技计划项目(2006；国家自然科学基金。 　　作者简介：刘刚（1983-），男，助教，硕士，研究方向：运动疗法研究。 　　 　　摘 要：为了探讨高强度训练对SD大鼠OAH品质的提高，将30只SD大鼠随机分为训练干预组与安静对照组。训练干预组以坡度10%、28 m/min、每次60 min、每天1次、每周5 d条件下于跑台上运动8周；安静对照组不施加运动干预措施。在最后一次训练结束72 h即刻进行腓肠肌与血清的取材。检测指标为骨骼肌与血清的丙二醛(malondialdehyde，MDA)、总超氧化物歧化酶(total super oxide dismutase，TSOD)、过氧化氢酶(catalase，CAT)和还原型谷胱甘肽(reduced glutathione，RGSH)。结果发现：骨骼肌与血清中的RGSH含量和TSOD活性显著性增高；MDA在血清中显著性降低，但在骨骼肌中显著性升高；CAT活性在血清中显著性增高，但??骨骼肌中不变。实验表明：高强度训练能够实现SD大鼠OAH升级。 　　关 键 词：运动生物化学；氧化还原；内稳态；高强度运动；大鼠 　　中图分类号：G804.7 文献标识码：A 文章编号：1006-7116(2012)02-0138-04 　　 　　Enhancement of oxidation?antioxidation homeostasis by high intensity exercising 　　LIU Gang1，PAN Hua-shan1，PENG Yi-hai2，FENG Yi-chong1，LIU Cheng-yi3 　　（1.School of Physical Education and Health，Guangzhou University of Chinese Medicine，Guangzhou 510006，China； 　　2.School of Physical Education，South China University of Technology，Guangzhou 510641，China； 　　3.Laboratory of Laser Sports Medicine，South China Normal University，Guangzhou 510006，China） 　　 　　Abstract: In order to probe into the enhancement of the quality of oxidant-antioxidant homeostasis (OAH) by high intensity exercising, the authors divided 30 SD rats randomly into a training intervention group and a calm control group. The rats in the training intervention group ran on a treadmill for 8 weeks under such conditions as 10% slope, 28m/min, 60min/time, 1 time/day, 5 days/week, while no exercising intervention measure was taken for the rats in the calm control group. Gastrocnemius muscle and blood serum were sampled at 72h after the last round of training. The test indexes were malondialdehyde (MDA), total superoxide dismutase (TSOD), catalase (CAT) and reduced glutathione (RGSH) in skeletal muscle and blood serum. The authors revealed the following findings: the content of RGSH and the activity of TSOD in skeletal muscle and blood serum increased significantly; MDA decreased significantly in blo