E-cad和PCNA在膀胱肿瘤中表达及其意义.docVIP

E-cad和PCNA在膀胱肿瘤中表达及其意义 　　[摘要] 目的 探讨上皮钙依赖黏附蛋白（E-cad）和增殖细胞核抗原（PCNA）在膀胱肿瘤中的表达及其意义。 方法 采用免疫组化SP法检测1995～2010年76例膀胱恶性肿瘤和12例良性肿瘤，计算每例切片中癌细胞E-cad的异常表达率及增殖细胞核抗原阳性指数（PLI）。 结果 E-cad在76例膀胱恶性肿瘤和12例膀胱良性肿瘤中异常表达率分别为84.2%、16.7%，差异有统计学意义（P ＜ 0.05）。膀胱良、恶性肿瘤中PLI增殖指数分别为（35.85±8.20）%、（80.24±19.52）%，差异有统计学意义（P ＜ 0.05）。两者在膀胱恶性肿瘤中表达呈正相关（P ＜ 0.05）。 结论 E-cad与膀胱恶性肿瘤的浸润、转移有关，可作为预测转移潜能的指标，PCNA可作为恶性肿瘤增殖的指标。 　　[关键词] 上皮钙依赖黏附蛋白；增殖细胞核抗原；膀胱肿瘤；免疫组织化学 　　[中图分类号] R737.14 [文献标识码] A [文章编号] 1674-4721（2012）02（b）-0096-02 　　 　　Expression and significance of E-cadherin (E-cad) and proliferating cell nuclear antigen (PCNA) in bladder tumor 　　GAO Fang1 HE Jinghua1 ZHANG Fengmei2 ZHANG Xiubin3 XU Hua2 LI Shengshui2 　　1.Department of Pathology, BOAI Hospital of Huanghua in Hebei Province, Huanghua 061100, China; 2.Department of Pathology, Integrated Chinese and Western Medicine Hospital of Cangzhou City in Hebe Province, Cangzhou 061000, China; 3.Department of Pathology, Nandagang Hospital in Hebei Province, Nandagang 061103, China 　　[Abstract] Objective To investigate the clinical significance of the expressions of E-cad and PCNA in bladder tumor. Methods Immunohistochemical study for E-cad and PCNA from 1995 to 2010 were performed on 76 cases of paraffin-embedded specimens of surgical carcinoma and 12 cases of mastoid tumor, the E-cad abnormal expression rate of cancer cells in slice of every patients and proliferating cell nuclear antigen positive index(PLI) were calculated. Results There were significant difference in E-cad and PCNA between the transitional cell carcinoma of bladder (84.2%) and mastoid tumor (16.7%). The positive expression of PCNA was significantly increased with stage and grade (P 　　1.4 统计学处理 　　采用SPSS 13.0统计学软件，阳性率之间的比较用χ2检验，两变量之间的关系用Spearman等级相关分析表示，检验水准α=0.05。 　　2 结果 　　2.1 E-cad和PCNA在膀胱肿瘤中的表达 　　E-cad在膀胱恶性肿瘤和良性肿瘤中的异常表达率分别为84.2%和16.7%，表达水平显著降低，差异有统计学意义（P ＜ 0.05）（表1）。PCNA阳性棕黄色颗粒位于肿瘤细胞的胞核。膀胱良、恶性肿瘤中PLI增殖指数分别为（35.85±8.20）%、（80.24±19.52）%，差异有统计学意义（P ＜ 0.05）（表1）。 　　2.2 E