心肌缺血后处理与缺血预适应相关性研究.docVIP

心肌缺血后处理与缺血预适应相关性研究 　　摘要：目的 在体条件下，比较缺血后处理与缺血预适应对大鼠心肌缺血再灌注损伤的保护作用，并观察两种保护性处理是否有叠加效应。方法 建立大鼠在体缺血再灌注模型，将30只大鼠随机分为假手术组、缺血再灌注组、缺血后处理组、缺血预适应组、缺血预适应加缺血后处理组，于再灌注末测定心肌组织梗死面积，测定心肌酶。结果 与缺血再灌注组相比，缺血后处理组、缺血预适应组、缺血后处理加缺血预适应组心肌梗死面积、血浆肌钙蛋白I明显降低（P 　　关键词：心肌；缺血-再灌注损伤；缺血后处理；缺血预适应 　　中图分类号：R542.2 R256.2 文献标识码：A 文章编号：1672-1349（2011）08-0971-03 　　 　　The Research for Relationship between Ischemic Postconditioning and Ischemic Preconditioning 　　Wei Xiaoxia，Xiao Chuanshi，Bian Yunfei 　　Department of Cardiology，The Second Hospital，Shanxi Medical University （Taiyuan 030001） 　　Abstract:Objective To compare the cardioprotective effects of ischemic postconditioning and ischemic preconditioning on ischemia-reperfusion（IR） injury of rats in vivo.Methods Thirty Wistar rats were randomly divided into five groups: Sham-operated group，IR group，ischemic postconditioning group，ischemic preconditioning group，ischemic preconditioning，and postconditioning group.The myocardial ischemia-reperfusion models of rats were established.Plasma cardiac treponin I （cTnI） were tested with biochemistry technique.Myocardial infarct size was measured by image analytical system.Results Compared with IR group，infarct size and the levels of cTnI were significantly less in postconditioning group，preconditioning group，preconditioning group and postconditioning groups.There were no differences between the three groups.Conclusion Ischemic postconditioning was as effective as ischemic preconditioning in reducing infarct size and serum cTnI.The combined protective effects did not appear to be additive，this probably meant that there was same or similar cardioprotective mechanism. 　　Key words:myocardium；ischemia reperfusion injury；ischemic postconditioning；ischemic preconditioning 　　 　　在心肌梗死、体外循环手术，器官移植等许多危重状态下，往往需要早期再灌注治疗，但是再灌注又可加重心肌的缺血损伤［1，2］，研究证实缺血预适应与缺血后处理均可显著减轻心肌缺血再灌注损伤。本实验利用大鼠在体心肌缺血再灌注模型，观察缺血后处理与缺血预适应对心肌的保护作用是否一致，两种保护措施同时合用是否有叠加效应。 　　1 材料与方法 　　1.1 药品与试剂、仪器 血浆肌酸激酶（CK）购自南京建成生物工程研究所。血浆肌钙蛋白I（cTnI）试剂盒购自美国LDI公司。Even’s blue染