番茄景天庚酮糖-1,7-二磷酸酯酶基因克隆及遗传转化-cloning and genetic transformation of sedum tomato heptyl ketose - 1,7 - diphosphate esterase gene.docxVIP

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番茄景天庚酮糖-1,7-二磷酸酯酶基因克隆及遗传转化-cloning and genetic transformation of sedum tomato heptyl ketose - 1,7 - diphosphate esterase gene.docx

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番茄景天庚酮糖-1,7-二磷酸酯酶基因克隆及遗传转化-cloning and genetic transformation of sedum tomato heptyl ketose - 1,7 - diphosphate esterase gene

番茄景天庚酮糖-1, 7-二磷酸酯酶基因的克隆及遗传转化also revealed that SBPase expression was higher in mid-position leaves than in base position leaves and upper position leaves. Measurements of Pn showed that the Pn of 8th apical leaves was higher than that of 4th, 12th, 1st and 16th leaves and the similar trend was observed in the changes of SBPase activity. The diurnal variations of gene expression at the mRNA level and SBPase activity in optimal functional leaf (8th leaf) was was similar to that of Pn, indicating the close relationship between SBPase and photosynthesis in tomato plants.Sense and anti-sense constructs were made by subcloning the full-length SBPase cDNA into the expression vector pBI121. The resulting plasmid was used for agrobacterium- mediated transformation of tomato plants. The successful transformants were screened and confirmed by real-time PCR and western blot. The sense transgenic plants showed the enhanced level of SBPase, while the antisense plants had decreased SBPase.Overexpression of SBPase led to increased SBPase mRNA abundance and activity. Compared with wild-type tomato plants, sense transgenic plants showed the enhanced Pn, increased accumulation of carbohydrate, widened stem, stronger root system, enlarged leaves and earlier flowering time. However, antisense suppression of SBPase in tomato plants led to the contrary changes to those by overexpression.SBPase expression in tomato plants was inhibited by low temperature stress, resulting in decreased mRNA level and enzyme activity, which significantly reduced the net photosynthesis and insignificantly affected the Fv/Fm and фPSII. In comparison with wild- type tomato plants, SBPase over-expressing plants had less reduction in Pn and EC under low temperature conditions, however, these plants showed no significant changes in Fv/Fm and фPSII. Anti-sense suppression of LeSBP led to significantly decreased Pn and increased EC in tomato plants. Our results showed that over-expression of SBPase increased Pn and