免疫化学 自噬LC3.pdfVIP

Virchows Arch (2011) 459:315–319 DOI 10.1007/s00428-011-1104-z ORIGINAL ARTICLE Immunohistochemical localization of autophagosomal membrane-associated protein LC3 in granular cell tumor and schwannoma Masayuki Shintaku Received: 16 March 2011 /Revised: 26 April 2011 /Accepted: 30 May 2011 /Published online: 15 June 2011 # The Author(s) 2011. This article is published with open access at S Abstract Granular cell tumor (GCT) is a neoplasm derived Introduction from Schwann cell or (in cases arising in the neurohypoph- ysis) pituicyte and is characterized by abundant cytoplasm Granular cell tumor (GCT) is a neoplasm with distinctive filled with numerous eosinophilic granules, which have histopathological and ultrastructural characteristics. It com- been considered autophagolysosomes on the basis of their monly arises in the skin and subcutaneous tissue, various ultrastructure. To confirm that the formation of these visceral organs, especially the breast and upper aerodigestive granules is related to an autophagy phenomenon, 12 cases tracts, and in the neurohypophysis [ 1]. Regarding the of GCT (including two cases of GCT of the neurohypoph- histogenesis of GCT, cases of GCT arising in the skin, ysis) were studied immunohistochemically using an anti- subcutaneous tissue, and visceral organs are widely believed body against LC3 (microtubule-associated protein 1 light to originate from Schwann cells [2–7]. The histogenesis of chain 3, a specific marker of autophagy). All cases of GCT GCT in the neurohypophysis remains controversial, but showed granular immunoreactivity for LC3 in the cyto- derivation from pituicyte has been proposed [8, 9]. Regard- plasm of tumor cells, indicating that the formati