甘蓝型油菜油脂功能相关分子图谱构建和含油量qtl定位-construction of molecular map related to oil function and qtl mapping of oil content in brassica napus l..docxVIP

杭州师范大学硕士学位论文甘蓝型油菜油脂功能相关分子图谱构建与含油量 杭州师范大学硕士学位论文 甘蓝型油菜油脂功能相关分子图谱构建与含油量 QTL 定位 IV IV Abstract Rapeseed is the third biggest source of vegetable oil, and one of the most important oilseed crops in China. Increasing the oil content of rapeseed is the major objective for breeders, and is an important approach to increase oil production. Oil content in rapeseed is a typical quantitative trait, which is controlled by multiple genes and affected by environment. Both the genetic basis of oil accumulation and the synthetic pathway of lipids are complicated in rapeseed. All of these bring great difficulty to breed varieties with high oil content. So it is necessary to combine molecular techniques with conventional breeding techniques. Therefore, to develop lipid related molecular functional markers, construct functional genetic map, analyse the QTL of oil content and their relationships with candidate genes, will facilitate the map based cloning and utilization of QTL to increase oil content in Brassica. napus. In addition, the comparative mapping between Brassica. napus and model plant Arabidopsis. thathaliana will accelerate the studies in rapeseed. In this study, an DH population of 282 lines resulting from crossing “Sollux × Gaoyou” was used for linkage analysis, and a function related genetic map was constructed using fuctional EST markers after sequence alignment between B. napus and A. thathaliana. Based on the map, QTL mapping was conducted for oil content by using field data in 9 different trials. Furthermore, the association between the functional markers and the QTL was analysed and the comparative mapping was done between B. napus and A. thathaliana. The major results are as follows: A total of 452 PCR primer pairs were designed according to the result of sequence alignment between B. napus and A. thathaliana based on the expressed genes. Of these, 121 primers can amplify clear, polymorphic band from the total genomic DNA of two parents. After tested in parents a