高产乙醛嗜热链球菌的筛选及其关键基因表达分析-screening of streptococcus thermophilus with high acetaldehyde yield and analysis of its key gene expression.docxVIP
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高产乙醛嗜热链球菌的筛选及其关键基因表达分析-screening of streptococcus thermophilus with high acetaldehyde yield and analysis of its key gene expression
目录1引言................................................................11.1发酵乳及发酵剂的概述............................................11.1.1发酵乳的概述..................................................11.1.2发酵剂的定义及其菌种种类......................................11.2嗜热链球菌的概述................................................21.2.1嗜热链球菌的生物学特性........................................21.2.2嗜热链球菌的应用..............................................21.3嗜热链球菌的产香特性............................................31.3.1发酵乳的风味物质..............................................31.3.2嗜热链球菌产乙醛的代谢途径....................................31.4实时荧光定量PCR技术的概述41.4.1实时荧光定量PCR技术的原理41.4.2实时荧光定量PCR的定量形式及应用51.5研究目的和内容..................................................61.5.1研究目的......................................................61.5.2研究内容......................................................62材料与方法..........................................................62.1试验材料........................................................62.1.1试验菌株......................................................62.1.2试验所用培养基及主要试剂......................................72.1.3试验器材......................................................72.2试验方法........................................................82.2.1菌株的活化培养及发酵剂的制备..................................82.2.2发酵特性的测定................................................82.2.3发酵乳样在贮藏期间风味的测定..................................92.2.4合成乙醛的关键功能基因的RT-PCR定量分析102.3统计分析及数据处理.............................................123结果分析与讨论.....................................................123.1发酵时间的测定.................................................123.2贮藏期间特性研究...............................................133.2.1滴定酸度与PH值的变化133.2.2贮藏期间活菌数的变化........................................163.3贮藏期间主要风味物质乙醛含量变化研究..........................173.3.1加标回收试验的结果..........................................173.3.2乙醛含量变化结果............................................183.4贮藏期间各种指标间的相关性分析................................213.5合成乙醛的关键功能基因的RT-PCR定量分析243.5.1RNA的检测图谱243
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