鸭场固废堆肥腐熟菌种的分子鉴定、优化及其应用分析-molecular identification, optimization and application analysis of decomposed bacteria from solid waste compost in duck farm.docxVIP

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鸭场固废堆肥腐熟菌种的分子鉴定、优化及其应用分析-molecular identification, optimization and application analysis of decomposed bacteria from solid waste compost in duck farm.docx

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鸭场固废堆肥腐熟菌种的分子鉴定、优化及其应用分析-molecular identification, optimization and application analysis of decomposed bacteria from solid waste compost in duck farm

PAGE PAGE III Abstract Compost is a common treatment for the waste of livestock, but it also have some disadvantages, for example, long time for fermentation and low quality. However, efficient composting inoculants are the key to solve this problem. Strains SR109 and DM109 were used in early pig manure compost research and good results were obtained. In this research, the strains SR109 and DM109 were used as starting strains, molecular biology identification was conducted, and then culuture conditions and carbon and nitrogen sources of two strains were optimized, at last, different doses of microorganism agent microorganism agent (consisted of strains SR109 and DM109) was used to solid waste of duck farm compost. 1 Molecular biology identification of compost strains. Genomic DNA of strains SR109 and DM109 was extracted by bacterial genomic DNA extraction kit, PCR amplification to get 16S rDNA sequences of two strains. PCR products were electrophoresed by 2% agarose gel, 16S rDNA sequences were recovery by gel extraction kit cwbiotech, and sequences were sequenced by Shanghai Sangon Biological Engineering Technology Services Co., Ltd., the sequence length of strain SR109 is 1437bp, the sequence length of strain DM109 is 1443bp. The sequencing results were submitted to the Genbank database, the accession numbers of SR109 and DM109 were JQ734474 and JQ734475, respectively. The sequences were analyzed by blast with Genbank for homology comparison, Mega4.0 software was used in phylogenetic analysis. The results showed SR109 has closest relationship with Bacillus vallismortis and Bacillus methylotrophicus, the homology was 100%, SR109 was belonged to Bacillus genus. DM109 had the closest relationship with Serratia grimesiiand Serratia proteamaculan, the homology was, 96%, DM109 was belonged to Serratia genus. Optimization of the cultivation condition of composting strains. In order to optimize strain SR109 and DM109 cultural condition, parameters like the c