万古霉素和液态庆大霉素复合骨水泥体外药物释放研究.docVIP

万古霉素和液态庆大霉素复合骨水泥体外药物释放研究 　　【摘要】目的探讨单独应用万古霉素骨水泥,万古霉素和液态庆大霉素复合使用的抗生素骨水泥药物释放的比较,为临床应用提供理论依据。 方法分别在40g骨水泥中加入1g万古霉素,1g万古霉素和160mg庆大霉素,用紫外分光光度仪测不同时间点各组的万古霉素释放浓度。 结果 载有与不载液态庆大霉素的万古霉素骨水泥药物的释放规律相似,载有液态庆大霉素和万古霉素的骨水泥使得万古霉素的释放率提高约51%。 结论 万古霉素和液态庆大霉素复合骨水泥有利于促进骨水泥中抗生素的释放。 　　【关键词】骨水泥;万古霉素;液态庆大霉素;释放 　　Drug release profile of Bone Cement incorporated with Vancomycin and Liquid Gentamicin 　　WeiZhao ShufengHAN Zhimin Tian 　　Department of Orthopedics the first hospital, Shanxi medical university, taiyuan 030001,china 　　【Abstract】Objective To compare the drug release of vancomycin bone cement with liquid gentamicin and without andtoprovide the theory basis for the clinical practice. Methods The specimens of bone cement each of 40g with 1g vancomycin were divided into 2 groups, group 1 with 160mg liquid gentamicin and group 2 without anything served as a control. The elution examination of vancomycin from bone cement was performed by UV spectrophotometry. Results The release rule of drug from the antibiotic bone cement with liquid gentamicin was similar with bone cement without gentamicin and vancomycin elution was enhanced by 51% with the addition of gentamicin liquid. Conclusion With liquid gentamicin in vancomycin bone cement are to be an effective additive to promote the release of antibiotics. 　　【Key words】Bone cement;Vancomycin;Liquid gentamicin;Release 　　 　　 载抗菌素的骨水泥已经被广泛的应用于临床治疗慢性骨髓炎,但其释放率偏低为 5%-8%[1]。持续低浓度释放可能使骨髓炎治疗效果差也可能引起耐药菌的产生。有报道[2]称载抗生素骨水泥的释放与骨水泥的孔隙率有关,加入可溶性的的添加剂可增加骨水泥的孔隙率进而促进抗生素的释放。本实验在体外加入液态庆大霉素,观察添加液态庆大霉素后对万古霉素释放的影响。 　　1材料与方法 　　1.1实验材料 　　 骨水泥(PMMA天津市合成材料工业研究所 ) ,万古霉素(美国礼来制药有限公司 0.5克),硫酸庆大霉素针剂,紫外分光光度计(uv-9600型北京瑞利仪器公司,100ml烧杯若干。 　　1.2实验方法 　　1.2.1制作含有万古霉素,万古霉素和庆大霉素两种抗生素混合体的骨水泥 样本分2组,每组10个标本。 　　Ⅰ组:40g骨水泥粉中加入1g万古霉素。 　　Ⅱ组:40g骨水泥中加入1g万古霉素和160mg的庆大霉素。 　　1. 2.2 干时在无菌条件下充分混匀,聚合反应后。在环境温度24℃和相对湿度不低于40%的无菌条件下,10个标本以自制模具模铸成直径(9.0±0.1) mm,高(10.0±0.1) mm的圆柱状骨水泥试件。贮于24℃干燥。 　　1.2.3 把做好的圆柱体抗生素骨水泥浸没于20ml的磷酸盐缓冲生理盐水(phosphate buffered saline,PBS液)中,水温37℃,每次取5ml样品取样时间分别为1d,2