家榆组织培养离体再生体系建立的初步研究.pdf

Abstract Linn．astheinitialmaterialWas Inthis andstemsofUlmus studied， study,seeds pumila accesstostablesterile the initial seedlings，andseedling throughcultureis，subculture，rooting were onthesterile and leaves、cotyledons、stemsseedling hardened、transplanted。Through adventitiousbuds intowhole itisfromoutdoorinduceanddifferentiatecallus．Then develop invitro ofUlmus canestablish genetically plants，we regenerationsystem pumila．Elm forthefuture theoreticalbasisfor and as cropsfactorynursery engineered provide improved mainresultswereasfollows： basematerial．The culture 1．Starting cottonwoolis Intheseed combinationofsterilewaterand sprout germinationstudy,the ofobtained stemInmid．Juneisthe inducedrate．Therate fastestof derived all；The highest was rate is17．6％． 76．8％，the plantlets ofbrowning culture 2．Primary suitableculturemediafortheseedsdifferentiationisMS+6-BA0．1mg·l’1+ Themost isMS+6-BA0．1 0．005 IBA0．025mg‘l～．Thebestmediumforbuds mg。l_+IBA growing differentiationrateof is is2．75 m