急性心肌梗死患者循环mirna-21 mirna-126的表达水平及其临床意义-expression level of circulating mirna - 21 mirna - 126 in patients with acute myocardial infarction and its clinical significance.docxVIP

diagnosis, evaluation, treatment of patients with acute myocardial infarction.The research which discusses that the miRNA - 21 and miRNA - 126 is associated with cardiovascular clinical has been carried out, but there is few sample for research analysis about the expression of circulating miRNA - 21 and miRNA – 126 in patients with acute myocardial infarction (mi).Objective:Whether the expression in circulating miRNA – 21 and miRNA – 126 has significant difference between the patients with acute myocardial infarction (mi) and the healthy people.Whether the expression in circulating miRNA – 21 and miRNA – 126 has significant difference between the groups at different time.The relationship between the expression levels of Circulating miRNA -21 and miRNA - 126 in patients who died of acute myocardial infarction (mi) and chest pain time, coronary Gensini score, LVEF value, MYO, CKMBII, cTnI, the WBC.Methods:Data collection: Choose 74 cases of patients with acute myocardial infarction in our hospital for treatment in March 2011 - February 2013 as observation group, choose 50 cases of healthy people in our hospital for check-up in March 2011 - February 2013 as control group, to extract elbow venous blood samples from above two groups of patients.Blood samples collection: The learning sample of patients who have a line of coronary angiography all be collected before undergoing coronary angiography. When we drawn the blood sample from the patients with acute myocardial infarction, we also record the time from the onset of chest pain in patients at the same time, and send parts of them to our clinical laboratory to test MYO (myoglobin), CKMBII (creatine kinase MB isoenzyme 2), cTnI (troponin I). Some patients tested the blood routine at the same time.We use quantitative reverse transcription-PCR (qRT-PCR) to detect the relativeexpression of circulating miRNA -21 and miRNA - 126, and make the following comparison by appropriate statistical methods.Using independent sample T t