美洛昔康对骨形态蛋白诱导间充质干细胞骨向分化的抑制作用.docVIP

人参皂甙Rg3抑制人结肠癌细胞生长与Wnt/β-catenin信号的关系研究 中国重庆， 摘 要：目的 研究对结肠癌细胞增殖抑制作用Wnt/β-catenin信号转导的关系 方法 结晶紫染色法检测对HCT116细胞增殖抑制作用β-catenin/Tcf4转录活性的影响。Western blot检测β-catenin蛋白；β-catenin核转移的抑制作用。 结果处理组细胞较对照组细胞增殖明显受到抑制。β-catenin/Tcf4的转录活性，并降低c-Myc蛋白表达。与对照组相比，Rg3能明显抑制SW480细胞中β-catenin核转移。结论 细胞增殖Wnt/β-catenin信号转导，其机制至少与β-catenin 核转移有关。 关键词：；；增殖抑制；β-catenin； Ginsenoside Rg3 Exerts Anti-proliferation Effect on Human Colon Cancer Cells via Wnt/β-catenin Signaling Pathway Abstract Aim To investigate correlation between the anti-proliferation effect of Rg3 on colon cancer cells and Wnt/β-catenin signaling. Methods Crystal violet staining and colon formation were employed to detect the anti-proliferation effect of Rg3 on HCT116 cells. The luciferase reporter assay was employed to measure the transcription activity of β-catenin/Tcf4. The expression of β-catenin and c-Myc was detected by western blot. Immunofluorescent assay was used to demonstrate the effect of Rg3 on the nuclear translocation of β-catenin in colon cancer cells. Results Rg3 can inhibit the proliferation and colony formation of HCT116 cells. The transcription activity of β-catenin/Tcf4 in HCT116 cells was inhibited by ginsenoside Rg3. The expression of c-Myc was decreased by Rg3, whereas Rg3 had no effect on the expression of β-catenin. The nuclear translocation of β-catenin was suppressed in SW480 cells. Conclusions Ginsenoside Rg3 can inhibit the proliferation of colon cancer cells and Wnt/β-catenin signaling transduction. This effect may be mediated at least by suppressing the nuclear translocation of β-catenin in colon cancer cells. Key words Ginsenoside Rg3; colon cancer; proliferation inhibition; β-catenin; nuclus translocation 结肠癌是一种常见癌症，其发病与遗传或某些管家基因突变有关[1]。人类结肠癌中有近80%与APC（adenomatous polyposis Coli，腺瘤性结肠息肉）突变有关，另外20%约一半与癌基因?-catenin突变有关。因此，异常活化的Wnt/?-catenin 信号在结肠癌的发生过程中具有生要作用[2,3,4]。近年来，由于对结肠癌发病机制的研究及诊断和治疗手段的发展，使得结肠癌的治疗有了很大的发展,但治疗效果仍不十分理想。传统的化疗药物多是增殖依赖的细胞毒性药物，具有较严重的不良反应。将传统化疗药物与其他非细胞毒性类天然化合物联合应用不仅能增强疗效，并且能