维胃方对乙酸诱导大鼠胃溃疡愈合效果及血清GAS，EGF水平的影响_论文.docxVIP

维胃方对乙酸诱导大鼠胃溃疡愈合效果及血清GAS，EGF水平的影响 【摘要】 目的探讨维胃方对消化性溃疡病愈合情况的影响及作用机制。方法采用乙酸诱导大鼠胃溃疡模型。观察维胃方对大鼠溃疡面积的改变；检测大鼠胃液pH值变化；通过石蜡包埋、病理切片染色观察溃疡愈合质量，并进行镜下分析；采用放射性免疫法（RIA）检测大鼠血清表皮生长因子（EGF）和胃泌素（GAS）水平的变化。结果①镜下可见维胃方治疗的大鼠胃黏膜造模处溃疡愈合情况较好，其中以中剂量组的效果最好；②模型组与各用药组比较，胃液pH值变化明显（P＜）；③而在溃疡面积的治疗效果比较中，维胃方各治疗组与对照组相比较有显著意义（P＜），其中以维胃方中剂量组值最小，疗效最好〔（±）mm2，P＜〕；④维胃方治疗的3组大鼠血清GAS均数都高于模型组和对照组，但其差异并无统计学意义；⑤而经维胃方治疗的大鼠血清EGF水平均有所降低，其中以维胃方中剂量组治疗效果最显著〔（ 5± 7 ng/ml)，P＜〕。结论维胃方对消化性溃疡有较好的治疗效果，其作用机制与防御因子GAS，EGF的变化有关系。 【关键词】 维胃方 消化性溃疡 表皮生长因子 胃泌素 溃疡愈合效果 Therapeutic Effect of WeiWeiFang(WWF) on Acetic Acid－induced Gastric Ulcer and the Levels of GAS and EGF in Rats 　Abstract:ObjectiveTo discuss the therapeutic effect and mechanism of WWF on peptic ulcer(PU). MethodsTo observe the change of ulcer area and pH of gastric juice,and analyse the slice up of pathology by the optics microscope by the method of acetic acid－inducted gastric ulcer. Levels of GAS and EGF serum were determined by specific radioimmunoassay ①The effect was significant when cured by WWF,especially in mid-dose group when observed under the optical microscope.②The change of pH of gastric juice was evident in model group compared with cured groups（P＜）.③ The difference was significant (P＜) while compare the curative effect on ulcer areas in control group of WFF with that in cured groups, and the effect was the best in the mid-dose group (P＜). ④ The levels of GAS in serum were higher in cured groups of WFF than those in model group and control group. But the difference was not significant. ⑤The level of EGF in WWF groups decreased,mid-dose group had the best effect（P＜〕.ConclusionThere is evident therapeutic effect of WWF on PU. And its mechanism is correlated to the defense of GAS and EGF. Key words:WeiWeiFang; Peptic ulcer; EGF; GAS; Effect of ulcer healing 消化性溃疡病（peptic ulcer，PU）是临床常见多发病。目前H2受体拮抗剂及质子泵抑制剂在临床上的广泛应用，使溃疡病的愈合率显著提高，但由于费用、副作用、耐药性、患者依从性等问题的存在，最终使该病的复发率仍居高不下。维胃方是经过临床应用的经验方， 对PU有较好的远期疗效。而胃泌素（GAS）和表皮生长因子（EGF）被认为是PU愈合过程中的防御因子，发挥着很重要的作用 ［1,2］。因此，