跑台训练增强大鼠学习记忆及其海马BDNF基因表达.docVIP

跑台训练增强大鼠学习记忆及其海马BDNF基因表达 　　摘 要： 目的：探讨8周跑台训练对大鼠学习记忆能力及其海马内脑源性神经营养因子（BDNF ）基因表达的影响。方法：将24只雄性SD大鼠分为对照组（C组，n=8）和训练组（T组，n=1 6），T组进行8周的跑台训练。通过Y迷宫实验，所有大鼠在第7周进行学习能力测试，在第8 周进行记忆能力测试。第8周最后一次训练后，随机将T组分为T1组（n=8）和T2组（n=8）。 T1组训练后即刻与C组同时断头处死，两组交叉进行，T2组在训练后24时断头处死，采用RT- PCR法检测各组大鼠海马内BDNF的基因表达水平。结果：Y迷宫测试表明T组大鼠的学习记忆 优于C组；与C组相比，T1组和T2组大鼠海马中BDNF的基因表达水平均显著升高（?p? 　　关键词：跑台训练；大鼠；学习记忆；海马；脑源性神经营养因子 　　中图分类号：G804.7文献标识码：A文章编 号：1007-3612(2011)04-0051-03 　　Research on the Enhancement of Treadmill Training on Learning?m emory and BDNF mRNA Expression in Hippocampus of Rats 　　 　　XU Bo，HUANG Tao， JI Liu 　　 （College of Physical Education and Health，East China Normal University，shangh ai，200241，China） 　　Abstract: Objective: The purpose of this study is to explore the effects of treadmill trai ning on learning?memory and BDNF mRNA expression in hippocampus of rats. Metho d: 24 male SD rats were assigned into two groups: control group (C，n=8)，traini ng group (T，n=16). The T group rats took part in treadmill training for 8 week s. In 7th week，all of the rats were participated the learning test by Y maze， and in 8th week，all of the rats were participated the memory test by Y maze. A fter the last treadmill training in 8th week，T group were assigned randomly in to two groups：T1 group(n=8) and T2 group(n=8)．The rats of the T1 group were de collated immediately after the last running alternately with the rats in controlgroup，and the rats of T2 group were decollated after 24 hours of the last runn ing．The expression of BDNF mRNA were inspected by RT?PCR.Results: Y maze testshowed that the ability of learning and memory for T group rats were better tha n the C group rats. Comparing with C group，the expression of BDNF mRNA increas ed significantly in T1 and T2 group（?p?［1,2］表明，适宜的游泳训练可提高大鼠的学习记忆能力。然 而， 运动??练影响学习记忆能力的机制尚不明确。近年来的研究表明，脑源性神经营养因子（br ain derived neurotrophic factor，BDNF）在中枢神经系统中具有广泛作用， BDNF与自主 跑轮运动引起的学习记忆能力的提高有一定的相关性［3,4］。目前，有关运动改善 学习记忆 能力的动物实验多采用自主跑轮运动（voluntary wheel running），其运动量不易控制。 有研究